Effects of Buyang Huanwu Decoction on mitochondrial oxidative damage and PKCε-Nampt pathway in rats following cerebral ischemia-reperfusion
10.3969/j.issn.1001-1528.2024.01.014
- VernacularTitle:补阳还五汤对脑缺血再灌注大鼠线粒体氧化损伤和PKCε-Nampt通路的影响
- Author:
Mei-Juan YIN
1
;
Zhen-Yi LIU
;
Xiao-Fei JIN
;
Xiao-Hong ZHOU
;
Yu GAO
;
Yue-Mou ZHAO
;
Wei-Juan GAO
Author Information
1. 河北中医学院, 河北省心脑血管病中医药防治研究重点实验室, 河北 石家庄 050091
- Keywords:
Buyang Huanwu Decoction;
cerebral ischemia-reperfusion injury;
mitochondria;
PKCε;
Nampt
- From:
Chinese Traditional Patent Medicine
2024;46(1):79-86
- CountryChina
- Language:Chinese
-
Abstract:
AIM To explore the effects of Buyang Huanwu Decoction on mitochondrial oxidative damage and PKCε-Nampt pathway in rats following cerebral ischemia reperfusion(I/R).METHODS The rats were randomly divided into the sham operation group,the model group,Buyang Huanwu Decoction group(14.3 g/kg)and edaravone group(3 mg/kg).Except those of the sham operation group,SD rats of other groups were induced into models of brain I/R injury by MCAO method,followed by corresponding drug administration 24 hours after operation.After 7 days of administration,the rats had their neurological deficit evaluated by neurological function scoring;thier expression of neuron marker MAP-2 detected by immunofluorescence staining;their neuron damage observed and the oxidative damage evaluated through assessment of their ROS levels and MDA and SOD activities;their changes of mitochondrial membrane potential detected by fluorescent probe JC-1;their ratio of NAD+/NADH detected using modified enzyme circulation method;their expressions of PKCε,p-PKCε and Nampt proteins detected with Western blot;and their positive expressions of p-PKCε and Nampt proteins detected with immunohistochemistry method.RESULTS Compared with the model group,Buyang Huanwu Decoction group shared decreased cerebral infarction volume and neurological function score(P<0.05);increased cerebral fluorescence intensity of MAP-2(P<0.05);reduced neuronal damage,decreased cerebral levels of ROS and MDA(P<0.05);increased SOD activity,mitochondrial membrane potential and NAD+/NADH ratio(P<0.05);and increased protein expressions of p-PKCε and Nampt(P<0.05).CONCLUSION Buyang Huanwu Decoction can improve mitochondrial function and reduce brain I/R injury in rats by activating their PKCε-Nampt signaling pathway.
