Analysis of component composition and content determination of six constituents for Xeriga-4 Powder
10.3969/j.issn.1001-1528.2024.01.004
- VernacularTitle:协日嘎-4味散成分组成分析及6种成分含量测定
- Author:
Jun LI
1
,
2
;
Yue-Wu WANG
;
Qian ZHANG
;
Ping CUI
;
Ri-Gui YE
;
Ji-ri-mu-ba-tu
Author Information
1. 内蒙古医科大学药学院, 内蒙古 呼和浩特 010110
2. 内蒙古医科大学新药筛选工程研究中心,内蒙古 呼和浩特 010110
- Keywords:
Xieriga-4 Powder;
component composition;
chemical constituents;
content determination;
high performance liquid chromatography-Q-exactive orbitrap mass spectrometry(HPLC-Q-Exactive-MS);
high performance liquid chromatography tandem mass spectrometry(HPLC-M
- From:
Chinese Traditional Patent Medicine
2024;46(1):14-22
- CountryChina
- Language:Chinese
-
Abstract:
AIM To analyze the component composition of Xeriga-4 Powder,and to determine the contents of phellodendrine,chlorogenic acid,gardenoside,berberine,rutin and curcumin.METHODS The high performance liquid chromatography-Q-exactive orbitrap mass spectrometry(HPLC-Q-Exactive-MS)qualitative analysis was performed on a 35℃thermostatic Agilent ZORBAX SB-Aq column(4.6 mm×150 mm,5 μm),with the mobile phase comprising of methanol-0.1%formic acid flowing at 0.35 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning.High performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)quantitative analysis was performed on a 35℃thermostatic Shim-pack GIST-HP C18 column(2.1 mm×100 mm,3 μm),with the mobile phase comprising of methanol-0.1%formic acid flowing at 0.25 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Total 65 constituents were identified,containing 19 alkaloids,13 organic acids,13 flavonoids,7 curcumins,6 iridoids,4 fatty acids,2 aldehydes,and 1 amino acid.Six constituents showed good linear relationships within their own ranges(r≥0.999 1),whose average recoveries were 96.44%-102.37%with the RSDs of 2.05%-3.74%.CONCLUSION This study can provide a reference for the quality control for Xieriga-4 Powder.
