Simultaneous content determination of twelve constituents in Bushen Huoxue Sanjie Capsules by HPLC

VernacularTitle:HPLC法同时测定补肾活血散结胶囊中12种成分的含量
Author: Ji-Yao YIN ¹ ; Jing HU ; Xia SHEN ; Xiao-Min CUI ; Hui REN ; Tong QU ; Ning LI ; Wen-Jin LU ; Zhi-Yong CHEN ; Kai QU
Author Information

1. 陕西中医药大学,陕西咸阳 712083
Keywords: Bushen Huoxue Sanjie Capsules; chemical constituents; content determination; HPLC
From: Chinese Traditional Patent Medicine 2024;46(1):1-6
CountryChina
Language:Chinese
Abstract: AIM To establish an HPLC method for the simultaneous content determination of gallic acid,protocatechuic acid,morroniside,loganin,sweroside,paeoniflorin,hypericin,astragalin,salvianolic acid B,salvianolic acid A,epimedin C and icariin in Bushen Huoxue Sanjie Capsules.METHODS The analysis was performed on a 30℃thermostatic Agilent 5 TC-C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 240 nm.RESULTS Twelve constituents showed good linear relationships within their own ranges(r≥0.999 8),whose average recoveries were 97.11%-101.14%with the RSDs of 0.60%-2.65%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Bushen Huoxue Sanjie Capsules.