Optimization of TLC identification for Cirsii Herba and research on changes in quality properties "carbonizing retains characteristics" of its charred product
10.3969/j.issn.1001-1528.2023.12.028
- VernacularTitle:小蓟TLC鉴别方法优化及小蓟炭"炒炭存性"内在表征研究
- Author:
Qi LU
1
,
2
;
Tian-Ze ZHANG
;
Juan-Juan ZHU
;
Zhen-Ni QU
;
Chun-Meng XU
;
Yan-Peng DAI
;
Dian-Hua SHI
Author Information
1. 山东省中医药研究院,山东 济南 250014
2. 国家中医药管理局中药蜜制和制炭炮制技术与原理重点研究室,山东 济南 250014
- Keywords:
Cirsii Herba;
Cirsii Herba Carbonisata;
TLC identification;
carbonizing retains characteristics
- From:
Chinese Traditional Patent Medicine
2023;45(12):4024-4029
- CountryChina
- Language:Chinese
-
Abstract:
AIM To optimize the TLC identification method for Cirsii Herba and to study the changes in quality properties"carbonizing retains characteristics"of Cirsii Herba Carbonisata.METHODS After the improvement of thin layer plate and solvent in the TLC identification based on the Chinese Pharmacopoeia 2020 Edition for Cirsii Herba,Cirsii Herba Carbonisata had its TLC identification method and UPLC fingerprint established for the determination of its content of buddleoside and acacetin as well.RESULTS We used silica gel G plate,and the solvent of toluene-acetone-formic acid-methanol(6 ∶ 3 ∶ 0.5 ∶ 2.5)for TLC identification of Cirsii Herba.The content variations of buddleoside and acacetin in Cirsii Herba and its differently charred products were consistent with the result of the TLC identification.CONCLUSION The improved TLC identification method for Cirsii Herba is of lower cost and less solvent toxicity compared to the method in the Chinese Pharmacopoeia 2020 Edition,and can identify the changes in quality properties"carbonizing retains characteristics"of differently charred Cirsii Herba,therefore it can be used to control the quality of Cirsii Herba Carbonisata in the market.
