UHPLC-Q-Orbitrap/MS-based analysis of saponins from Panacis Quinquefolii Radix of different origins
10.3969/j.issn.1001-1528.2023.12.027
- VernacularTitle:基于UHPLC-Q-Orbitrap/MS的不同产地西洋参皂苷类成分分析
- Author:
Li-Wen LIANG
1
;
Na GUO
;
Xiao-Kang LIU
;
Xin HUANG
;
Guang-Zhi CAI
;
Yun-Long GUO
;
Ji-Yu GONG
Author Information
1. 长春中医药大学药学院,吉林 长春 130117
- Keywords:
Panacis Quinquefolii Radix;
saponin;
principal component analysis;
orthogonal partial least-squares discriminant analysis;
differential component analysis;
UHPLC-Q-Orbitrap/MS
- From:
Chinese Traditional Patent Medicine
2023;45(12):4017-4024
- CountryChina
- Language:Chinese
-
Abstract:
AIM To analyze saponins from Panacis Quinquefolii Radix of different regions(Jilin,Liaoning,Heilongjiang,Shandong)based on UHPLC-Q-Orbitrap/MS technology.METHODS Panacis Quinquefolii Radix had its saponins qualitatively analyzed by UHPLC-Q-Orbitrap/MS;and its differential saponins revealing different regions screened by the principal component analysis,orthogonal partial least squares discriminant analysis and differential component analysis.RESULTS A total of 62 saponins were identified;saponin variations due to the growth areas verified by the principal component analysis;and 28 differential saponins including 13 protopanaxadiol,6 protopanaxatriol,4 oleanolane,2 oxytetracycline,and 3 C-17 side chain variants further screened by orthogonal partial least-squares discriminant analysis model.The relative contents of protopanaxadiol-type and protopanaxatriol-type saponins in the four producing areas reduced following the order of Liaoning,Jilin,Heilongjiang,and Shandong.CONCLUSION This simple,accurate and efficient method provides a theoretical basis for the quality evaluation of Panacis Quinquefolii Radix.
