Isolation and identification of dominant strains in the fermentation process of HuaFengDan YaoMu and investigation of their growth under different temperature and pH value
10.3969/j.issn.1001-1528.2023.12.026
- VernacularTitle:化风丹药母发酵过程中优势菌种分离鉴定及不同温度和pH值下菌株生长情况
- Author:
Guo-Qiong CAO
1
,
2
,
3
;
Min LIU
;
Yao LIU
;
Jian XU
;
You-Li CHEN
;
Jing-Lan WU
;
Yong-Ping ZHANG
Author Information
1. 贵州中医药大学药学院,贵州 贵阳 550025
2. 国家苗药工程技术研究中心,贵州 贵阳 550025
3. 贵州中药炮制与制剂工程技术研究中心,贵州 贵阳 550025
- Keywords:
HuaFengDan YaoMu;
isolation and identification;
Bacillus aryabhattai;
Debaryomyces hansenii;
Aspergillus oryzae
- From:
Chinese Traditional Patent Medicine
2023;45(12):4011-4017
- CountryChina
- Language:Chinese
-
Abstract:
AIM To isolate and identify the dominant strains in the fermentation process of the HuaFengDan YaoMu,and to investigate the effects of different temperatures and pH values upon their growth.METHODS During the 0-6 weeks fermentation process of HuaFengDan YaoMu in the selective medium,the cultured bacteria,the molds and the yeasts had the colonies counted,and the dominant strains isolated and purified,particularly the dominant bacteria and fungi determined by 16S rDNA and 26S rDNA sequences.The optimal growth temperature and pH value of Bacillus aryabhattai,Debaryomyces hansenii,Aspergillus oryzae were investigated respectively.RESULTS HuaFengDan YaoMu found its bacterial counts increased from 0 to 4 weeks and stabilized from 4 to 6 weeks;its yeast counts increased during 0-3 weeks,and decreased continuously during 4-6 weeks;and its mold counts remained unchanged at 0 week,but increased steadily from 1 to 4 weeks,and decreased at 5 to 6 weeks of the fermentation.In the fermentation process of HuaFengDan YaoMu,the dominant strains of Bacillus aryabhattai,Debaryomyces hansenii and Aspergillus oryzae,identified and screened out by the phylogenetic tree,adapted best to the growth temperature of 35℃,25-30℃and 35℃,at pH value of 8,6 and 7,respectively.CONCLUSION In the fermentation process of HuaFengDan YaoMu,the dominant microorganisms of Bacillus aryabhattai,Debaryomyces hansenii,Aspergillus oryzae are identifiable and separable.
