Effects of rhubarb ethanol extract on hemin-induced autophagy and apoptosis of HT22 cells
10.3969/j.issn.1001-1528.2023.12.012
- VernacularTitle:大黄醇提物对氯化血红素诱导HT22细胞自噬和凋亡的影响
- Author:
Meng-Ru YUE
1
;
Kun WANG
;
Xiao-Ning JI
;
Yan-Hua YANG
;
Zhen LEI
;
Ming MA
;
Xu ZHAO
Author Information
1. 河南中医药大学药学院,河南 郑州 450046
- Keywords:
ethanol extract of rhubarb;
HT22 cells;
hemin;
cerebral hemorrhage;
autophagy;
apoptosis
- From:
Chinese Traditional Patent Medicine
2023;45(12):3930-3936
- CountryChina
- Language:Chinese
-
Abstract:
AIM To explore the effects of rhubarb ethanol extract on autophagy and apoptosis of HT22 cells after hemin-induced injury.METHODS The HT22 cells were divided into the blank group,the model group,and the high,medium and low dose rhubarb ethanol extract groups(25,12.5 and 6.25 μg/mL).After the 24 hours corresponding drug intervention,3 hours induction of 40 μmol/L hemin was used for the modeling of cell cerebral hemorrhage injury.Subsequently,the cells had their cell viability detected by CCK-8 method;their leakage rate of lactate dehydrogenase(LDH)detected by kit;their apoptosis rate detected by flow cytometry;and their apoptosis and autophagy related protein expressions of cysteine protease-3(caspase-3),Bcl-2 related X protein(Bax),B cell lymphoma-2(Bcl-2)and microtubule related protein light chain 3(LC3)detected by Western blot.RESULTS Compared with the normal group,the model group displayed decreased cell viability(P<0.01);increased LDH leakage rate,apoptosis rate and the protein expressions of Bax/Bcl-2,caspase-3,Beclin1 and LC3(P<0.01);and decreased protein expression of P62(P<0.01).Compared with the model group,all of the groups intervened with rhubarb ethanol extract showed increased cell viability(P<0.05,P<0.01),decreased LDH leakage rate,apoptosis rate and protein expressions of Bax/Bcl-2,caspase-3,Beclin1 and LC3(P<0.05,P<0.01),in addition to the decreased protein expression of P62 increased(P<0.05,P<0.01)in the high and middle dose rhubarb ethanol extract groups.CONCLUSION The ethanol extract of rhubarb may protect neurons and reduce cell damage through its efficacy in regulating their autophagy and apoptosis.
