Simultaneous determination of six constituents in Xiao'er Qingyan Granules by HPLC
10.3969/j.issn.1001-1528.2023.12.008
- VernacularTitle:HPLC法同时测定小儿清咽颗粒中6种成分
- Author:
Yan HE
1
;
Xiao-Xiang HU
;
Yang-Jia LIU
;
Rui DAI
;
Jia-Qi TANG
;
Hui ZHANG
Author Information
1. 湘南学院药学院,湖南 郴州 423000
- Keywords:
Xiao'er Qingyan Granules;
chemical constituents;
HPLC
- From:
Chinese Traditional Patent Medicine
2023;45(12):3906-3909
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of monocaffeyltartaric acid,forsythiaside A,chicoric acid,phillyrin,arctiin and harpagoside in Waigan Fenghan Granules.METHODS The analysis of 70%methanol extract of this drug was performed on a 30℃thermostatic Agilent Zorbax SB-C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 278,330 nm.RESULTS Six constituents showed good linear relationships within their own ranges(r≥0.999 4),whose average recoveries were 96.80%-102.61%with the RSDs of 0.26%-1.93%.CONCLUSION This simple and accurate method can be used for the quality control of Xiao'er Qingyan Granules.
