Simultaneous determination of fifteen constituents in Zhuriheng Dropping Pills by UPLC-MS/MS
10.3969/j.issn.1001-1528.2023.12.005
- VernacularTitle:UPLC-MS/MS法同时测定朱日亨滴丸中15种成分
- Author:
Ren BU
1
;
Peng-Peng ZHANG
;
Yue-Wu WANG
;
Wen ZU
;
Fang-Yuan LIANG
;
Xiao-Ning WANG
;
Jing-Kun LU
Author Information
1. 内蒙古医科大学药学院,内蒙古 呼和浩特 010010
- Keywords:
Zhuriheng Dripping Pills;
chemical constituents;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2023;45(12):3890-3894
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of ellagic acid,gallic acid,dehydrodiisoeugenol,methyleugenol,agarotetrol,elemicin,chlorogenic acid,geniposide,rutin,ferulic acid,hydroysafflor-yellow A,deoxycholic acid,cholic acid,borneol and taurine in Zhuriheng Dropping Pills.METHODS The analysis of 50%methanol solution of this drug was performed on a 30℃thermostatic Thermo C18 column(2.1 mm×100 mm,1.9 μm),with the mobile phase comprising of acetonitrile-0.1%formic acid(containing 10 mmol/L ammonium acetate)flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning.RESULTS Fifteen constituents showed good linear relationships within their own ranges(R2>0.990 0),whose average recoveries were 75.91%-112.13%with the RSDs of 3.06%-10.66%.CONCLUSION This simple,sensitive and efficient method can be used for the quality control of Zhuriheng Dropping Pills.
