Loss of primary cilia promotes migration of TPC-1 in papillary thyroid cancers
10.3760/cma.j.cn311282-20230615-00272
- VernacularTitle:miR-182-5p调控初级纤毛的缺失增加甲状腺乳头状癌细胞系TPC-1的迁移
- Author:
Chengxu MA
1
;
Xiaoni MA
;
Lihua MA
;
Songbo FU
Author Information
1. 兰州大学第一临床医学院，兰州大学第一医院内分泌科，兰州 730000
- Keywords:
Papillary thyroid carcinoma;
Migration;
Primary cilia;
miR-182-5p;
TPC-1
- From:
Chinese Journal of Endocrinology and Metabolism
2024;40(2):158-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of miR-182-5p regulated primary cilia loss on migration of TPC-1 in papillary thyroid cancers(PTC).Methods:Ten cases of PTC and adjacent tissues were collected from the First Hospital of Lanzhou University, The expression of miR-182-5p in PTC tissue and TPC-1 cells was detected by qPCR, and the frequency of primary cilia was detected by immunofluorescence; Overexpressing miR-182-5p, the migrated number of TPC-1 was detected by Transwell assay; Interfering TPC-1 with siRNA-IFT88, the migrated number of TPC-1 and the frequency of primary cilia were detected, respectively.Results:Compared with control, the expression of miR-182-5p was significantly upregulated in PTC and TPC-1, the frequency of primary cilia in PTC and TPC-1 was downregulated. Overexpressing miR-182-5p increased the migrated number of TPC-1 cell and reduced the number of TPC-1 cell migration(27%, P=0.002); After siRNA-IFT88 treatment, primary cilia in TPC-1 became shorter and thinner, with a decrease in frequency( P=0.001), the migrated number of TPC-1 cell increased, and TPC-1 cell showed smaller nuclei and fewer microvilli. Conclusion:The regulation of primary cilia loss by miR-182-5p through the PI3K pathway contributes to the migration of TPC-1 cells. The loss of primary cilia has an adverse impact on the prognosis of PTC.
