Metabolic mechanisms of thyroid cancer in different background using ultra-high performance liquid chromatography combined with mixed four-stage poles time-of-flight mass spectrometry
10.3760/cma.j.cn311282-20230118-00035
- VernacularTitle:基于超高效液相色谱混合四级杆飞行时间质谱技术的代谢组学探讨不同背景下甲状腺癌的代谢机制
- Author:
Danyang SUN
1
;
Yujie ZHANG
;
Xue LI
;
Dan WANG
;
Rui HAN
;
Ning LI
;
Tingwei LI
;
Xue ZHAO
;
Qiang JIA
;
Jian TAN
;
Wei ZHENG
;
Lili SONG
;
Zhaowei MENG
Author Information
1. 天津医科大学总医院空港医院核医学科，天津 300308
- Keywords:
Metabolomics;
Hashimoto′s thyroiditis;
Papillary thyroid cancer;
Metabolism pathway;
Biomarkers
- From:
Chinese Journal of Endocrinology and Metabolism
2023;39(9):751-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the metabolic mechanism of papillary thyroid cancer(PTC) in normal and Hashimoto′s thyroiditis(HT) background, and to explore the relationship between HT and PTC.Methods:This study included a matched sample set collected from Tianjin Medical University General Hospital between January 2018 and January 2019, consisting of PTC and paracancular tissue from 31 cases with coexisting HT(HT group), and 30 cases without(NC group), all confirmed pathologically following thyroidectomy. The ultra-high performance liquid chromatography combined with mixed four-stage poles time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was employed to acquire data from the samples. Metabolite differences between the two groups were compared, aiming to identify distinct metabolic mechanisms of PTC under different backgrounds. Metabolic pathway analysis was conducted using Metabo-Analyst 5.0 to explore relevant metabolic pathways.Results:The HT group and NC group shared 7 common differentially expressed metabolites, including arginine, glutamic acid, cysteine, citric acid, malic acid, uracil, and taurine. Logistic regression model combined with receiver operating characteristic(ROC) analysis of these 7 biomarkers yielded excellent discriminatory capacity for PTC(area under ROC curve of HT group and NC group were 0.867 and 0.973, respectively). The common metabolic pathways were taurine and hypotaurine metabolism, arginine biosynthesis, alanine, aspartic acid and glutamic acid metabolism, arginine and proline metabolism, and glutamine and glutamic acid metabolism. The specific metabolic pathways in HT group were aminoacyl tRNA biosynthesis, glycine, serine, and threonine metabolism.Conclusion:The metabolic profiles of thyroid cancer exhibit significant differences between cases with normal backgrounds and those with HT. The specific pathways for PTC and HT are aminoacyl tRNA biosynthesis and the metabolism of glycine, serine, and threonine.
