Effect of sleep deprivation on expression of SIRT6 in cerebellum of immature mice
10.3760/cma.j.cn131073.20230927.00109
- VernacularTitle:睡眠剥夺对幼鼠小脑沉默信息调节因子6表达的影响
- Author:
Lan XIAO
1
;
Chenyi GAO
;
Ting CHEN
;
Xuemin ZHANG
;
Qi ZHONG
;
Yue CAO
;
Lijuan TANG
;
Chang CHEN
;
Zongze ZHANG
Author Information
1. 武汉大学中南医院麻醉科，武汉 430071
- Keywords:
Sleep deprivation;
Cerebellum;
Silent information regulator 6
- From:
Chinese Journal of Anesthesiology
2024;44(1):41-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of sleep deprivation on the expression of sirtuin 6 (SIRT6) in the cerebellum of immature mice.Methods:Fifty SPF healthy male C57BL/6 mice, aged 4 weeks, weighing 14-16 g, were divided into 2 groups ( n=25 each) using a random number table method: control group (Con group) and sleep deprivation group (SD group). The chronic sleep deprivation model was prepared by using the multi-platform water environment method, with 20 h of sleep deprivation per day for 10 consecutive days. After sleep deprivation, a balance beam experiment was performed to test the balance and coordination ability of mice. The mice were sacrificed after anesthesia and cerebellar lobular IV-VI (4-6 cb) tissues were taken for microscopic examination of the ultrastructure (with a transmission electron microscope) and for determination of the dendritic spine density of cerebellar 4-6cb Purkinje neurons (by Golgi staining), co-expression of SIRT6 and Calbindin D-28k (CbD-28k) and expression of glucose transporter Glut3 of cerebellar 4-6cb (by immunofluorescence staining). Results:Compared with group Con, the duration of passage through the balance beam was significantly prolonged, and the number of posterior foot slips was increased, the synaptic gap of cerebellar 4-6cb neurons was increased, the thickness of postsynaptic density was increased, the density of dendritic spines of Purkinje cells and the number of positive cells co-expressing SIRT6 and CbD-28k were decreased, and the expression of Glut3 was down-regulated in group SD ( P<0.05). Conclusions:The mechanism by which sleep deprivation decreases the abilities of balance and coordination is related to down-regulating SIRT6 expression in cerebellar Purkinje cells and decreasing neuronal glucose metabolism, thus damaging the synaptic plasticity of cerebellum in immature mice.
