Comparison of effects of propofol, fospropofol disodium and sevoflurane on pulmonary metastasis after radical mastectomy for breast cancer in mice
10.3760/cma.j.cn131073.20230602.00910
- VernacularTitle:丙泊酚/磷丙泊酚二钠/七氟烷对小鼠乳腺癌根治术肺转移影响的比较
- Author:
Bichen HE
1
;
Chao WANG
;
Zhen YUE
;
Xiaohu NI
;
Bing ZHANG
Author Information
1. 新疆医科大学附属肿瘤医院麻醉与围术期医学中心，乌鲁木齐 830000
- Keywords:
Sevoflurane;
Propofol;
Breast neoplasms;
Lung;
Neoplasm metastasis
- From:
Chinese Journal of Anesthesiology
2023;43(9):1072-1075
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the effects of propofol, fospropofol disodium and sevoflurane on pulmonary metastasis after radical mastectomy for breast cancer in mice.Methods:SPF-grade healthy female C57 mice, aged 4-6 weeks, weighing 14-18 g, were used in this study. Eighteen mouse breast cancer models were successfully prepared by luciferase-labeled mouse 4T1 breast cancer cells, and the mice were divided into 3 groups ( n=6 each) by the random number table method: sevoflurane group (S group), propofol group (P group) and fospropofol disodium group (PD group). Group S inhaled 3% sevoflurane, propofol 200 mg/kg was intraperitoneally infused in group P, and fospropofol disodium 182 mg/kg was injected via the tail vein in group PD, and anesthesia was maintained for 3 h to perform radical mastectomy in three groups. The lung metastasis of mouse breast cancer cells was evaluated by in vivo imaging at 2 weeks after surgery, and then the mice were sacrificed, and lung tissues were obtained for determination of the expression of extracellular regulatory protein kinase 1/2 (ERK1/2), phosphorylated ERK1/2 (p-ERK1/2), P38 and phosphorylated P38 (p-P38) by Western blot. Results:Compared with S group, the number of lung metastases and total number of cell metastases in breast cancer cells were significantly decreased, and the expression of p-ERK1/2 and p-P38 in lung metastases was down-regulated in P group and PD group ( P<0.05). There was no significant difference in the parameters mentioned above between P group and PD group ( P>0.05). Conclusions:Propofol and fospropofol disodium have better efficacy than sevoflurane in reducing lung metastasis after radical mastectomy, which may be related to down-regulation of p-ERK1/2 and p-P38 expression in mice.
