Effects of cleavage factor Im25 downregulation in vascular smooth muscle cells on hyperlipidemia in mice
10.3760/cma.j.issn.0254-9026.2023.09.015
- VernacularTitle:剪切因子Im25在血管平滑肌细胞中的缺失对小鼠高脂血症的影响
- Author:
Qingbao LI
1
;
Yu WANG
;
Kefeng YE
;
Xinxin LI
;
Zitong YAO
;
Lei JIANG
;
Jingjing HUANG
Author Information
1. 山东第一医科大学附属省立医院心外科，济南
- Keywords:
Hyperlipidemia;
Cleavage stimulation factor;
Muscle, smooth, vascular
- From:
Chinese Journal of Geriatrics
2023;42(9):1105-1109
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the impact of cleavage factor Im25(CFIm25)on VSMCs-specific knockdown in the context of hyperlipidemia.Methods:Mice models were constructed with specific knockout of CFIm25 in VSMCs(CFIm25f/+ TaglnCre)and control mice(TaglnCre).The mice were fed a normal diet or high-fat diet(HFD)for 18 weeks and their body weight changes were monitored.ELISA was used to measure serum total cholesterol(TC), triacylglycerol(TG), high-density lipoprotein(HDL-C)and low-density lipoprotein(LDL-C)levels.The extent of aortic lipid deposition in mice was assessed by oil red O staining.Results:During the feeding of a high-fat diet, CFIm25f/+ TaglnCre mice showed a significant increase in body weight compared to the control group[Male(1.01±0.06)g and(0.87±0.31)g, t=7.53, P<0.05; Female: (0.64±0.02)g and(0.35±0.04)g, t=9.68, P<0.05].After 18 weeks of high-fat diet feeding, CFIm25f/+ TaglnCre mice had significantly higher levels of TC[(6.80±0.35)mmol/L and(3.76±0.87)mmol/L, t=5.63, P=0.004], TG[(0.97±0.21)mmol/L and(0.42±0.10)mmol/L, t=4.08, P=0.015], and LDL-C[(5.20±0.30)mmol/L and(2.00±0.98)mmol/L, t=5.40, P=0.006]compared to the TaglnCre group.Specifically, TC levels increased by 80.72%, TG increased by 132.79%, and LDL-C increased by 160.32%.There was a significant increase in aorta lipid deposition and atherosclerotic plaque area in CFIm25f/+ TaglnCre mice( P<0.05). Conclusions:The research indicated that VSMCs-specific CFIm25 knockdown in mice further worsened hyperlipidemia and atherosclerotic lesions.
