Modulation of hypoxia inducible factor-1 alpha in neurons after cerebral hypoxia by recombinant adenovirus-mediated hypoxia inducible factor-1 alpha
10.3760/cma.j.issn.0254-9026.2023.08.015
- VernacularTitle:重组腺病毒介导的缺氧诱导因子-1α对脑缺氧后神经元中缺氧诱导因子-1α的调控
- Author:
Zhehua ZOU
1
;
Tao TAO
;
Minglu JIN
;
Jian XU
Author Information
1. 秦皇岛市第一医院全科医疗科，秦皇岛 066000
- Keywords:
Adenoviridae infections;
Hypoxia-inducible factor 1, alpha subunit;
Hypoxia, brain
- From:
Chinese Journal of Geriatrics
2023;42(8):970-973
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulatory effects and possible mechanisms of recombinant adenovirus-mediated hypoxia inducible factor-1 alpha(HIF-1α)transfection on HIF-1α in post-hypoxic rat cortical neurons.Methods:(1)Preparation of rats' cerebral cortical neurons in primary culture and hypoxia model.Recombinant adenovirus-mediated HIF-1α(AdHIF-1α)transfection and recombinant Ad transduction in normal and hypoxic cells.Then they will be divided into normal control group, hypoxia group, the recombinant adenovirus hypoxia-inducible factor-1α(AdHIF-1α)gene transfection group and recombinant adenovirus empty vector(Ad)transfection group.(2)To observe the transfection of AdHIF-1α/ Ad under fluorescence microscopy.The expression of hypoxia-inducible factor-1 alpha(HIF-1α)were observed by Western blot analysis in each group of neurons at 12 h、24 h、48 h and 72 h.Results:Hypoxic neurons transfected with Ad/AdHIF-lα showed the obvious expression 48 h under fluorescence microscopy.At each time of the AdHIF-1α gene transfection group, the expression of HIF-1α is significantly increased, the positive expression emerges at 12 h, the expression increased at 24 h, and it reaches the peak at 48 h, until 72 h declines, and it has statistical significance compared with the hypoxia group( P<0.01, P<0.05), but the Ad group has no statistically significant differences compared with the hypoxia group. Conclusions:The transfection of recombinant adenovirus-mediated HIF-1α gene can significantly increase the expression levels of HIF-1α in hypoxic neurons, and it may play a protective role in the neurons after hypoxia.
