Component-resolved diagnosis using microarray for diagnosing hypersensitivity to raw fruits in birch pollen sensitized children.
10.4168/aard.2015.3.3.200
- Author:
Won Bok CHOI
1
;
Jueng Sup YOU
;
Yoon Young YI
;
Soo In JEONG
;
Joon Sup SONG
;
Seong YANG
;
Il Tae HWANG
;
Ha Baik LEE
;
Hey Sung BAEK
Author Information
1. Department of Pediatrics, Hallym University Kangdong Sacred Heart Hospital, Seoul, Korea. paviola7@hanmail.net
- Publication Type:Original Article
- Keywords:
Allergen component;
Fruit allergy;
Birch allergy;
Microarray
- MeSH:
Antibodies;
Betula*;
Child*;
Diagnosis*;
Fruit*;
Humans;
Hypersensitivity*;
Immunoglobulin E;
Pollen*;
Prunus persica;
Rhinitis, Allergic, Seasonal
- From:Allergy, Asthma & Respiratory Disease
2015;3(3):200-205
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Recently, component-resolved diagnosis (CRD) using microarray technology has been introduced to the field of clinical allergy. This study was aimed to investigate the clinical usefulness of microarray-based IgE detection for diagnosing clinical raw fruit allergy in birch pollen-sensitized children. METHODS: Thirty-one children with allergic disease who had been sensitized to pollen were studied. A pollen-sensitized patient was defined as having an allergen-specific history with concomitant positive skin-prick tests (SPTs) to natural allergen extracts or positive allergen-specific IgE. All subjects underwent SPTs for pollen and fruit. In all subjects, specific IgE to pollen and fruit were measured by ImmunoCAP. Specific IgE antibodies to allergen components were determined by a customized allergen microarray (ISAC). RESULTS: Thirteen of the 31 patients (41.9%) had a history of fruit hypersensitivity with positive SPTs. Measuring IgE to allergen components by ISAC, all the 13 patients with fruit hypersensitivity were positive to at least one of Mal d 1, Pru p 1, Pru p 3, Act d 8, and Act d 2 compared to 12 of the 13 patients (92.3%) who had at least 1 positive IgE to fruits (apple, peach, and kiwi) using ImmunoCAP. The sensitivity of ISAC microarray was 100.0% for the diagnosis of fruit hypersensitivity, but its specificity was 27.7% (5/18). The sensitivity of ImmunoCAP was 92.3%, and its specificity was 83.3%. CONCLUSION: The sensitivity of allergen components tested using microarray for the diagnosis of clinical fruit hypersensitivity in children with pollen allergy was high; however, its specificity was low.
