Animal experimental study of biliary fibrosis in end to end anastomosis after transection of the common bile duct
10.3760/cma.j.cn113884-20230205-00029
- VernacularTitle:胆管对端吻合术后胆管纤维化的动物实验研究
- Author:
Yilei DENG
1
;
Yafei ZHANG
;
Pengfei MA
;
Wenjie MA
;
Rongxing ZHOU
;
Longshuan ZHAO
Author Information
1. 郑州大学第一附属医院肝胆胰外科，郑州 450000
- Keywords:
Common bile duct;
Transection;
Anastomosis;
Fibrosis
- From:
Chinese Journal of Hepatobiliary Surgery
2023;29(10):748-752
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of biliary fibrosis after end to end anastomosis of bile duct.Methods:12 Neijiang miniature pigs (6-8 months old, weight 30-40 kg) were divided into experimental group ( n=6) and control group ( n=6). The model of end to end anastomosis after transection of the common bile duct was established in experimental group. Control group was a sham operation group, and only T tube was placed. After 9 months, Masson staining, fluorescence quantitative PCR and immunohistochemistry were used to analyze the expressing changes of pro-fibrotic factor transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF) and epithelial-mesenchymal transition (EMT) markers, including cytokeratin-19 (CK19), E-cadherin (E-Cad) and fibroblast specific protein-1 (S100A4), α-smooth muscle actin (α-SMA) and collagen components Collagen I (COL-1), collagen III (COL-3) and fibronectin (FN) in the anastomotic bile duct tissues. Results:Masson staining showed that the submucosal collagen fibers increased significantly in the experimental group. Compared with the control group, the mRNA expression of TGF-β1 [(3.482±0.313) vs. (1.000±0.102), t=18.43, P<0.001], CTGF [(2.160±0.287) vs. (1.000±0.103), t=9.32, P<0.001] were increased, the difference was statistically significant. Compared with control group, the mRNA and protein expression of CK19 and E-Cad were decreased in the experimental group, while the mRNA and protein expression of S100A4 and α-SMA were increased in the experimental group (all P<0.01). Conclusion:It was feasible in the short term to perform an end-to-end anastomosis after transection of the common bile duct, but there was an obvious fibrosis in the anastomotic bile duct tissue at later time.
