Study on the mechanism of heterogeneous nuclear ribonucleoprotein L promoting the proliferation of hepatocellular carcinoma cells
10.3760/cma.j.cn113884-20230411-00111
- VernacularTitle:异质核核糖核蛋白L促进肝细胞癌细胞增殖的机制研究
- Author:
Jiaxin CHEN
1
;
Song HU
;
Fubin LIU
;
Zhenwei MA
;
Kang YANG
;
Shengquan ZOU
;
Fei XIONG
;
Bing WANG
Author Information
1. 鄂州市中心医院普外一科，鄂州 436099
- Keywords:
Carcinoma, hepatocellular;
Heterogeneous nuclear ribonucleoprotein L;
Proliferation
- From:
Chinese Journal of Hepatobiliary Surgery
2023;29(9):694-699
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of HNRNPL protein on the proliferative ability of primary hepatocellular carcinoma cells and its potential mechanism.Methods:Online public database and real-time quantitative PCR were used to analyze the difference of HNRNPL expression between cancer and adjacent tissues. The effects of HNRNPL on HCC cell MHCC97H and HepG2 proliferation and MAPK pathway were investigated by Western blot, cell counting assay, colony formation assay and nude mouse transplantation tumor experiments.Results:The level of HNRNPL mRNA was validated to be higher in HCC tissue (2.76±0.37) than in normal tissue (1.00±0.14) with statistical difference ( t=3.93, P=0.002). Colony formation assay showed that the colony numbers of two MHCC97H knockdown groups (33.3±7.7) and (43.3±2.2) were lower than their control group (84.3±6.2), and two HepG2 knockdown groups (59.0±15.5) and (41.7±4.8) were lower than their control group (200.3±6.2) with statistical difference (both P<0.01). HNRNPL knockdown decreased the proliferation ability and activation level of MAPK pathway in HCC cells. Overexpression of oncogene c-RAF partially alleviated the anti-proliferation effect of HNRNPL knockdown and rescued the tumorigenic capacity. Conclusion:HNRNPL can promote hepatocellular carcinoma cell proliferation by activating MAPK signaling pathway.
