Study on immunogenic cell death related proteins in nasopharyngeal carcinoma patients before and after radiotherapy
10.3760/cma.j.cn113030-20221217-00423
- VernacularTitle:鼻咽癌患者放疗前后免疫原性细胞死亡相关蛋白的研究
- Author:
Jinhua LONG
1
;
Lu XU
;
Weili WU
;
Xiuling LUO
;
Xiaoxiao CHEN
;
Cui LONG
;
Linmei ZENG
;
Xianhuai JIN
;
Wei WANG
;
Chunyan SHAO
;
Wei XIONG
;
Feng JIN
;
Zhu ZENG
Author Information
1. 贵州医科大学附属肿瘤医院头颈肿瘤科，贵阳 550004
- Keywords:
Nasopharyngeal carcinoma;
Immunogenic cell death;
Calreticulin;
Heat shock protein 70;
High mobility group protein box-1
- From:
Chinese Journal of Radiation Oncology
2023;32(10):886-891
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of clinical conventional fractionated dose radiation on the expression levels of immunogenic cell death (ICD) related proteins in patients with nasopharyngeal carcinoma (NPC).Methods:A total of 38 newly-treated NPC patients admitted to the Affiliated Cancer Hospital of Guizhou Medical University from November 2020 to December 2021 were enrolled, all of whom received induction chemotherapy and concurrent chemoradiotherapy, and another 20 healthy volunteers were selected as controls for a prospective study. The contents of ICD related proteins, namely calreticulin (CRT), high mobility group box 1 protein (HMGB-1) and heat shock protein 70 (HSP70) and the proportion of dendritic cell (DC) in the peripheral blood of patients were detected before treatment, after induction chemotherapy and after concurrent chemoradiotherapy, respectively. The correlation between the above indicators, general clinical data and short-term efficacy was analyzed by statistical methods such as t-test and analysis of variance (ANOVA). Results:The levels of HSP70 and HMGB-1 in peripheral blood of NPC patients before treatment were higher than those of healthy controls (both P<0.05). After concurrent chemoradiotherapy, the content of CRT was significantly higher than that before treatment ( P<0.05), whereas the difference before and after induction chemotherapy and the difference before and after concurrent chemoradiotherapy were not significantly correlated with the short-term efficacy of NPC patients. HSP70 level was significantly decreased after concurrent chemoradiotherapy ( P<0.001). There were no significant differences in the content of HMGB-1 after induction chemotherapy and concurrent chemoradiotherapy (both P>0.05). Conclusion:NPC patients receiving TPF regimen (docetaxel+cisplatin+fluorouracil) for induction chemotherapy and sequential cisplatin concurrent chemotherapy may induce ICD in NPC cells, and CRT has potential value in reflecting the clinical efficacy of NPC.
