Effects and mechanism of ultra-high dose rate irradiation in reducing radiation damage to zebrafish embryos
10.3760/cma.j.cn112271-20231023-00135
- VernacularTitle:超高剂量率照射减轻斑马鱼胚胎放射损伤的作用及机制研究
- Author:
Hui LUO
1
;
Leijie MA
;
Ronghu MAO
;
Na LI
;
Fumin XUE
;
Xuenan WANG
;
Hongchang LEI
;
Shuai SONG
;
Hong GE
;
Chengliang YANG
Author Information
1. 郑州大学附属肿瘤医院肿瘤放疗科，郑州 450008
- Keywords:
Ultra-high dose rate irradiation;
Zebrafish;
Embryo;
Oxidative stress;
Radiation damage
- From:
Chinese Journal of Radiological Medicine and Protection
2024;44(3):174-180
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To conduct a comparative analysis of the radiation damage to zebrafish embryos and the associated biological mechanism after ultra-high dose rate (FLASH) and conventional dose rate irradiation.Methods:Zebrafish embryos at 4 h post-fertilization were exposed to conventional and FLASH irradiation (9 MeV electron beam). The mortality and hatchability of zebrafish after radiation exposure were recorded. Larvae at 96 h post-irradiation underwent morphological scoring, testing of reactive oxygen species (ROS) levels, and analysis of changes in oxidative stress indicators.Results:Electron beam irradiation at doses of 2-12 Gy exerted subtle effects on the mortality and hatchability of zebrafish embryos. However, single high-dose irradiation (≥ 6 Gy) could lead to developmental malformation of larvae, with conventional irradiation showing the most significant effects ( t = 0.87-9.75, P < 0.05). In contrast, after FLASH irradiation (≥ 6 Gy), the ROS levels in zebrafish and its oxidative stress indicators including superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were significantly reduced ( t = 0.42-15.19, P < 0.05). There was no statistically significant difference in ROS levels in incubating solutions after conventional and FLASH irradiation ( P > 0.05). Conclusions:Compared to conventional irradiation, FLASH irradiation can reduce radiation damage to zebrafish embryos, and this is in a dose-dependent manner. The two irradiation modes lead to different oxidative stress levels in zebrafish, which might be a significant factor in the reduction of radiation damage with FLASH irradiation.
