Effects of glycosylated nanoparticles on radiation-induced the polarization of macrophages in early injured lung tissue
10.3760/cma.j.cn112271-20230220-00172
- VernacularTitle:糖基纳米颗粒对辐射诱导的早期肺组织巨噬细胞极化的影响研究
- Author:
Kejun LI
1
;
Liqing DU
;
Qiang LIU
;
Huijuan SONG
Author Information
1. 中国医学科学院北京协和医学院放射医学研究所，天津 300192
- Keywords:
Radiation-induced lung injury;
Pulmonary macrophages;
M2 polarization;
Reactive oxygen species;
Inflammation
- From:
Chinese Journal of Radiological Medicine and Protection
2023;43(11):858-865
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of glycosylated nanoparticles on early radiation responses including the generation of reactive oxygen species (ROS), the polarization of pulmonary macrophages, and secretion of inflammatory cytokines in mice lung tissues.Methods:Twenty mice were randomly divided into control group, drug administration group, irradiation group and irradiation + drug administration group. The irradiation group and irradiation + drug administration group were subjected to whole lung irradiation with X-rays. The antioxidant ability of glycosylated nanoparticles was characterized using ROS indicator (CM-H 2DCFDA). The M2 polarization of pulmonary macrophages was detected by flow cytometry and PCR. The mRNA and protein expression levels of inflammatory cytokines were investigated by PCR and ELISA assay, respectively. Results:Compared with the irradiation group, the intensity of ROS fluorescence signals was significantly lower ( t=15.76, P < 0.05), the proportion of M2-type macrophages was significantly higher ( t=2.89, P < 0.05), the expression level of arginase 1 (ARG-1) gene was elevated, and the expression levels of tumour necrosis factor α (TNF-α), interleukin 1β (IL-1β) and interleukin 6 (IL-6) inflammatory factors were significantly reduced ( t=3.32, 2.90, 2.85, 4.55, 2.88, P < 0.05) in the lung tissues of irradiation + treatment group. Conclusions:Glycosylated nanoparticles can effectively scavenge ROS, trigger polarization of M2 macrophage, dampen inflammatory responses, and thus potentially alleviate radiation-induced lung injury.
