Comparative analysis of the temporal characteristics of plasma metabolites in glioma mice after ultra-high dose rate radiation and conventional radiation
10.3760/cma.j.cn112271-20230330-00102
- VernacularTitle:超高剂量率照射与常规照射后胶质瘤小鼠血浆代谢物时序性特征对比分析
- Author:
Jun ZHANG
1
;
Wentao HU
;
Zhiming XU
;
Xinyang CHENG
;
Yongsheng ZHANG
;
Zhifei CAO
Author Information
1. 苏州大学附属第二医院病理科，苏州 215004
- Keywords:
Ultra-high dose rate radiation;
Conventional radiation;
Glioma;
Biomarker
- From:
Chinese Journal of Radiological Medicine and Protection
2023;43(10):759-765
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of ultra-high dose rate radiation (FLASH-RT) and conventional radiation (CONV-RT) on plasma metabolites in glioma mice.Methods:Tocally 21 male C57BL/6J mice bearing intracranial glioma xenograft were randomly divided into healthy control group ( n=3), CONV-RT group ( n=9) and FLASH-RT group ( n=9). The CONV-RT group was administered a single dose of 24 Gy radiation on the head of mice at a dose rate of 0.4 Gy/s, and the FLASH-RT group was administered a single dose of 24 Gy radiation on the head of mice at a dose rate of 60 Gy/s, and the healthy control group was given 0 Gy pseudoradiation under the same condition. Mice blood was collected through the inner canthus vein for plasma separation at 1, 3 and 7 d after radiation in the two radiation groups, and the blood plasma of healthy control group was collected at 7 days after sham radiation. The changes in plasma metabolites were detected by the non-targeted metabolomics based on liquid chromatography mass spectrometry tandem platform. Results:After irradiation, the metabolites in plasma of two irradiation groups had significant difference. Compared with the healthy control group, 12 and 5 differential metabolites were screened out in the FLASH-RT group and CONV-RT group at three time points, respectively. The difference of plasma metabolites had the largest value at 1 day and decreased at 3 and 7 d after radiation. The arachidonic acid and isovaleric acid, involving arachidonic acid metabolism, biosynthesis of unsaturated fatty acids, and tyrosine metabolism pathways were screened in both FLASH-RT group and CONV-RT group, and the 10 differential metabolites, mainly involving energy metabolism and redox reactions, only existed in the FLASH-RT group.Conclusions:Arachidonic acid and isovaleric acid may be the common sensitive biomarkers to different radiation patterns, which provides ideas for further exploring the molecular mechanism of FLASH-RT in the treatment of glioma.
