Investigation of the effects and mechanisms of berberine on a mouse model of polycystic ovary syndrome: based on intestinal flora analysis
10.3760/cma.j.cn112141-20231222-00272
- VernacularTitle:小檗碱对多囊卵巢综合征小鼠模型的作用及其机制基于肠道菌群分析
- Author:
Ge XIN
1
;
Lingyun ZHANG
;
Hongrong QIU
;
Yangchun WANG
;
Yuhong SUI
;
Baigong XUE
;
Honglei WANG
Author Information
1. 长春工业大学化学与生命科学学院食品科学与工程系，长春 130012
- Keywords:
Polycystic ovary syndrome;
Berberine;
Gastrointestinal microbiome;
Disease models, animal
- From:
Chinese Journal of Obstetrics and Gynecology
2024;59(3):215-226
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To examine the impact of berberine on polycystic ovary syndrome (PCOS) in mice, and to investigate the effects of berberine on the intestinal flora and the intestinal flora on PCOS.Methods:A mouse model of PCOS was established by administering dehydroepiandrosterone in combination with high fat diet, and the mouse model was given a berberine treatment. The study consisted of a blank control group (C group), a PCOS model group (M group) and a berberine treatment group (T group). During the experiment, the mice were closely monitored through timed body weight measurements and estrous cycle monitoring; intraperitoneal glucose tolerance test and insulin tolerance test were done. Upon completion of the pharmacological intervention, the wet weights of liver, ovary and fat deposits of mice were assessed and subjected to HE staining to confirm the success of PCOS modeling and the efficacy of berberine. Additionally, fecal samples were analyzed for intestinal flora through 16S rRNA analysis.Results:The PCOS model was established successfully, berberine alleviated the disturbance of estrous cycle in mice, and significantly alleviated fat accumulation and metabolic abnormalities of glucose in mice. The cross-sectional area of fat pad cells in T group was (2 858±146) μm2, which was significantly lower than that in M group [(9 518±347) μm2], and the difference was statistically significant ( P<0.001). The blood glucose levels in T group were significantly lower than those in M group ( P<0.05). The composition and structure of intestinal flora in mice of M group with PCOS (compared with C group) and in mice of T group after berberine intervention (compared with M group) were significantly altered. However, alpha diversity did not change significantly among three groups ( P>0.05). Conclusion:Berberine could alleviate PCOS by intervening in the alterations of gut microbiota.
