Optimization of Phenylphthalein and Phenolic Acid Components Extracts from Angelicae Sinensis Radix-Chuanxiaong Rhizoma Decoction Pieces by Central Combination Design Response Surface Methodology
10.19879/j.cnki.1005-5304.202310526
- VernacularTitle:中心组合设计-响应面法优选当归-川芎饮片苯酞、酚酸类成分提取工艺
- Author:
Qin SU
1
;
Qiang BAO
;
Xixiang LI
;
Xuemei WANG
;
Runliang YIN
;
Jianliang WANG
Author Information
1. 甘肃中医药大学药学院,甘肃 兰州 730000
- Keywords:
Angelicae Sinensis Radix-Chuanxiaong Rhizoma decoction pieces;
phthalide components;
phenolic acid components;
central combination design-response surface methodology;
extraction process
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(4):146-152
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the optimal extraction process for phthalein and phenolic acid components in Angelicae Sinensis Radix-Chuanxiaong Rhizoma decoction pieces.Methods On the basis of a single factor experiment,central combination design-response surface methodology was adopted,and the extraction time,ethanol concentration,and ethanol dosage were used as influencing factors,and the total normalized values of the content of Senkyunolide I,Senkyunolide A and ligustilide,and extract yield were used as evaluation indicators to optimize the extraction process of phthalein components in Angelicae Sinensis Radix-Chuanxiaong Rhizoma decoction pieces;the total normalized values of chlorogenic acid,caffeic acid,ferulic acid,and extract yield were used as evaluation indicators to optimize the extraction process of phenolic acids components in Angelicae Sinensis Radix-Chuanxiaong Rhizoma decoction pieces.Results The optimal extraction process was to add 7 times the amount of 90%ethanol to the phthalein components,extract for 130 minutes each time,and extract twice;phenolic acid components were extracted twice with 7.5 times the amount of 65%ethanol for 120 minutes each time.Conclusion The optimized extraction process for phthalein and phenolic acids in Angelicae Sinensis Radix-Chuanxiaong Rhizoma decoction pieces is stable and feasible,which can provide a basis for subsequent research.
