Isolation of Endophytic Fungi from Angelica sinensis and Evaluation of Their Secondary Metabolite Activities
10.19879/j.cnki.1005-5304.202308441
- VernacularTitle:当归内生真菌分离及其次级代谢产物活性评价
- Author:
Yujie ZHAO
1
;
Nan WANG
;
Ming HUANG
;
Zongyuan WANG
;
Yajuan DU
;
Xiaoxiao ZHAO
;
Yang LI
;
Jiamei PEI
;
Kailin CHEN
;
Bei WANG
;
Xinguo ZHANG
Author Information
1. 兰州理工大学生命科学与工程学院,甘肃省中藏药筛选评价及深加工重点实验室,甘肃 兰州 730050
- Keywords:
Angelica sinensis;
rhizosphere soil;
endophytic fungi;
isolation;
identification;
enzyme inhibitory activity;
bacteriostatic activity
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(4):139-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate endophytic fungi from Angelica sinensis and evaluate the bioactivity of their secondary metabolites.Methods Angelica sinensis and rhizosphere soil were utilized as materials.The tissue homogenization method was employed with six diverse culture media to isolate endophytic fungi.The antibacterial activity of secondary metabolites was gauged using a 96-well plate assay,while UV spectrophotometry was used to evaluate the inhibitory activity of four enzymes.Results A total of 153 fungal strains were isolated and purified from Angelica sinensis roots,stems,leaves,and soil.The samples exhibited specific inhibitory activities against adenosine deaminase(ADA),β-lactamase,xanthine oxidase(XO),and tyrosinase(TYR),with rates of 45.83%,52.78%,51.39%and 55.56%,respectively.Furthermore,1.39%of the samples displayed wide-ranging inhibitory effects against four indicator bacteria.Strain 6B also showcased the lowest inhibitory concentration values of 62.5 and 7.81 μg/mL against Escherichia coli ATCC25922 and ATCC35218,respectively,signifying its potential research significance.Conclusion Angelica sinensis has abundant endophytic fungal resources and is a good source for discovering active compounds,demonstrating certain research value.
