Simultaneous Determination of 9 Components in Shujin Tongluo Black Plaster by HPLC
10.19879/j.cnki.1005-5304.202309561
- VernacularTitle:HPLC同时测定舒筋通络黑膏药9个成分含量
- Author:
Jianliang WANG
1
;
Xixiang LI
;
Yali ZHOU
;
Xiaofeng LI
;
Qin SU
;
Runliang YIN
Author Information
1. 甘肃中医药大学药学院,甘肃 兰州 730000
- Keywords:
Shujin Tongluo Black Plaster;
chemical composition;
HPLC;
α-obscurine;
simultaneous determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(3):138-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC-based method for the determination of α-obscurine,ferulic acid,hydroxysafflower yellow A,benzoylneaconitine,benzoylaconitine,periplosin,4-methoxysalicylsalicylate,kaempferol and oleanolic acid simultaneously in Shujin Tongluo Black Plaster.Methods HPLC method was used to determine the 9 components in Shujin Tongluo Black Plaster simultaneously.The 70%methanol extracts were analyzed using Waters SunFire C18 chromatography column(4.6 mm×250 mm,5 μm),in mobile phase containing acetonitrile-0.2%phosphoric acid solution for gradient elution with volume flow rate of 1.0 mL/min;column temperature was set at 25℃;detection wavelength was set at 230 nm.Results The 9 components had good linear relationship in their respective ranges(r≥0.999 7).The average recoveries were between 98.06%-100.56%and RSD was between 0.48%-2.56%,respectively.Conclusion The method has the advantage of repeatability,simple and fast,and can be used as the quality control of Shujin Tongluo Black Plaster.
