Effects of Mild Moxibustion on Proteins Expression of BDNF/TrkB Signaling Pathway in Hypothalamus of Primary Dysmenorrhea Rats with Cold and Dampness Stagnation Syndrome
10.19879/j.cnki.1005-5304.202304679
- VernacularTitle:温和灸对寒湿凝滞型原发性痛经大鼠下丘脑BDNF/TrkB信号通路蛋白表达的影响
- Author:
Xiangyun MENG
1
;
Fei GAO
;
Di WANG
;
Yulei LIANG
;
Xuan ZHANG
;
Yanqiu SUN
;
Yanxue XING
;
Zhiguo ZHAO
;
Xinhua LI
Author Information
1. 河北中医药大学,河北 石家庄 050200
- Keywords:
primary dysmenorrhea;
cold and dampness stagnation syndrome;
mild moxibustion;
pain;
c-fos;
BDNF/TrkB signaling pathway;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(3):127-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the analgesic effects of mild moxibustion on primary dysmenorrhea(PD)rats with cold and dampness stagnation syndrome and its effect on BDNF/TrkB signaling pathway in hypothalamus;To explore its mechanism for the treatment of PD.Methods A total of 32 Wistar non-pregnant female rats were randomly divided into blank group,model group,Western medicine group and mild moxibustion group,with 8 rats in each group.Except for the blank group,the other groups received estradiol benzoate intraperitoneal injection combined with ice bath treatment + oxytocin intraperitoneal injection to establish PD with cold and dampness stagnation syndrome model.The mild moxibustion group received treatment at"Shenque"and"Guanyuan"from the eighth day of modeling for 10 min,and the Western medicine group was given ibuprofen solution intragastically for 4 days.The latency period of rats twisting was observed and the twisting score was calculated,Western blot and PCR were used to detect the expressions of c-fos,BDNF,TrkB protein and mRNA in hypothalamic tissue.Results Compared with the blank group,the model group showed a shortened latency period and an increased twisting score(P<0.01),the expressions of c-fos,BDNF,TrkB protein and mRNA in hypothalamic tissue increased(P<0.01,P<0.05).Compared with the model group,the mild moxibustion group had a longer latency period and lower twisting score(P<0.01),while the expressions of c-fos,BDNF,TrkB protein and mRNA in hypothalamic tissue increased(P<0.01,P<0.05).Conclusion Mild moxibustion may effectively improve the pain state of PD rats with cold and dampness stagnation syndrome.This mechanism may be related to downregulating c-fos expression,inhibiting BDNF/TrkB signaling pathway activation,thereby inhibiting pain signal transmission,regulating pain occurrence and maintenance.
