Exploration on Mechanism of Baihu Decoction in Treating Acute Lung Injury Based on Network Pharmacology,Molecular Docking and Experimental Verification
10.19879/j.cnki.1005-5304.202303696
- VernacularTitle:基于网络药理学、分子对接和实验验证探讨白虎汤治疗急性肺损伤作用机制
- Author:
Zhuannan HU
1
;
Jiajun CHEN
;
Xianwei WU
;
Wei ZHOU
;
Lei QIU
;
Shaoyan ZHANG
;
Zhenhui LU
Author Information
1. 上海中医药大学附属龙华医院呼吸疾病研究所,上海 200030
- Keywords:
Baihu Decoction;
acute lung injury;
network pharmacology;
experimental verification;
mechanism
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(3):19-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Baihu Decoction in the treatment of acute lung injury based on network pharmacology and molecular docking technology;To carry out experimental verification.Methods The active components and targets of Baihu Decoction were searched through TCMSP and BATMAN-TCM databases,and human gene searches were conducted in GeneCards,NCBI,and OMIM databases.PPI network construction and GO and KEGG pathway enrichment analysis were conducted to determine the important signaling pathways of Baihu Decoction and acute lung injury.Molecular docking of main active components and core target proteins was performed.The effects of Baihu Decoction on survival rate and inflammatory cytokine content in acute lung injury lethal model mice were observed through animal experiments.Results Totally 211 common targets for Baihu Decoction and acute lung injury were screened,and identified effective components such as quercetin,kaempferol,and stigmasterol,etc.Analysis of KEGG pathway enrichment indicated that Baihu Decoction exerted its pharmacological effects in acute lung injury through a variety of signal pathways,including Toll-like receptor signaling pathway,NOD-like receptor signaling pathway,T cell receptor signaling pathway,and MAPK signaling pathway.Molecular docking results showed that Baihu Decoction had good binding strength with MAPK14,STAT3,JUN,MAPK1,MAPK3,FOS and RELA.The results of animal experiments showed that compared with the model group,the survival rate of mice in the Baihu Decoction group was significantly increased,the degree of pathological injury in the lung tissue was reduced,and serum IL-6,TNF-α contents decreased significantly(P<0.05).Conclusion Baihu Decoction can treat acute lung injury by reducing pathological injury to lung tissue and releasing of inflammatory factors.
