Effects of Xixin Decoction on Blood-brain Barrier Permeability and Expressions of P-gp,CB1 and CB2 Proteins in Hippocampal Tissue of SAMP8 mice
10.19879/j.cnki.1005-5304.202305150
- VernacularTitle:洗心汤对快速老化模型小鼠血脑屏障通透性及海马组织P-gp、CB1、CB2蛋白表达的影响
- Author:
Xinyue HAN
1
;
Yongchang DIWU
;
Liqi DUAN
;
Enlong ZHAO
;
Hu ZHANG
;
Yali WANG
;
Yuan ZHOU
Author Information
1. 陕西中医药大学第一临床医学院,陕西 咸阳 712046
- Keywords:
Xixin Decoction;
Alzheimer disease;
blood-brain barrier;
SAMP8
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(2):110-115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Xixin Decoction on the blood-brain barrier permeability and the expressions of P-glycoprotein(P-gp),cannabinoid receptor 1(CB1)and cannabinoid receptor 2(CB2)in hippocampal tissue of rapidly aging mice(SAMP8);To explore the possible mechanism of Xixin Decoction in the treatment of Alzheimer disease(AD).Methods Totally 60 SAMP8 mice were randomly divided into model group,probiotics group,and Xixin Decoction high-,medium-and low-dosage groups,with 12 mice in each group,another 12 SAMR1 mice were set as control group.The medicated groups received corresponding drugs by gavage for 10 weeks respectively,while the control group and model group were gavaged with equal volume of distilled water.Morris water maze test was used to detect the learning and memory ability of mice,the blood-brain barrier permeability was detected by Evans blue method,the contents of matrix metalloproteinase 9(MMP9),nuclear factor(NF)-κB and tumor necrosis factor-α(TNF-α)in serum were determined by ELISA,the expressions of P-gp,CB1 and CB2 in hippocampal tissue were detected by Western blot,P-gp expression in hippocampal tissue was detected by immunofluorescence staining.Results Compared with the control group,the learning and memory ability of mice in model group significantly decreased,Evans blue exudation in brain tissue significantly increased,the contents of MMP9,TNF-α and NF-κB in serum significantly increased,the expressions of P-gp and CB2 protein significantly decreased,the expression of CB1 protein significantly increased,with statistical significance(P<0.01,P<0.05).Compared with the model group,the learning and memory ability of mice in Xixin Decoction high-dosage group significantly increased,the Evans blue exudation in brain tissue significantly decreased,the contents of MMP9,TNF-α and NF-κB in serum significantly decreased,the protein expressions of P-gp and CB2 significantly increased,and the protein expression of CB1 significantly decreased,with statistical significance(P<0.01,P<0.05).Conclusion Xixin Decoction can improve the spatial learning and memory ability of AD model mice,and its mechanism is related to regulating the permeability of the blood-brain barrier and related protein expression,and inhibiting neuroinflammation.
