Effects of Bushen Zhuyun Prescription on Endometrial Angiogenesis by Regulating Mitochondrial Function
10.19879/j.cnki.1005-5304.202303041
- VernacularTitle:补肾助孕方调节线粒体功能对子宫内膜血管生成的影响
- Author:
Yuling ZHOU
1
;
Jinglei ZHANG
;
Can CAO
;
Ying SUN
;
Ruobing NIU
;
Yingmei ZHANG
;
Shuchan HU
;
Lin LUO
;
Ming HE
Author Information
1. 河北中医药大学中西医结合学院(研究所),河北省中西医结合肝肾病证研究重点实验室,河北省中西医结合生殖疾病协同创新中心,河北 石家庄 050200
- Keywords:
Bushen Zhuyun Prescription;
kidney governing reproduction;
mitochondria;
angiogenesis;
endomentrial receptivity;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(1):91-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Bushen Zhuyun Prescription on regulating mitochondrial function and endometrial angiogenesis;To explore its mechanism of improving endometrial receptivity.Methods The mouse model of controlled ovarian hyperstimulation(COH)was established,and the mice were randomly divided into normal group,model group,and Bushen group,with 20 mice in each group.Bushen group received Bushen Zhuyun Prescription for gavage for 11 d,and the normal group and model group received normal saline for gavage.The number of embryo implantation was counted,the endometrial morphology was observed by HE staining,α-smooth muscle actin(α-SMA)expression was observed by immunofluorescence staining.Human endometrial microvascular endothelial cells(HEMECs)were cultured in vitro,they were divided into control group,VEGFA group,Bushen group and VEGFA + Bushen group,and were intervened with VEGFA and/or Bushen Zhuyun Prescription medicated serum.The activities of mitochondrial respiratory chain complexes Ⅰ-Ⅳ,the content of ATP,the expression of PCNA and Caspase-3 were detected.Results Animal experiment showed that,compared with the normal group,the number of embryo implantation in model group significantly decreased(P<0.05),α-SMA protein expression in endometrial tissue significantly decreased(P<0.05);compared with the model group,the number of embryo implantation in Bushen group significantly increased(P<0.05),α-SMA protein expression in endometrial tissue significantly increased(P<0.05).Cell experiment showed that,Bushen Zhuyun Prescription medicated serum could increase the activity of mitochondrial respiratory chain complexes Ⅰ-Ⅳ and ATP content in HEMECs,promote PCNA protein expression,and inhibit Caspase-3 protein expression(P<0.05,P<0.01).Conclusion Bushen Zhuyun Prescription can promote endometrial angiogenesis through improving mitochondrial function.
