Time-course analysis of DNA damage response-related genes after in vitro radiation in H460 and H1229 lung cancer cell lines.
10.3858/emm.2011.43.7.046
- Author:
Kang Ho KIM
1
;
Hae Yong YOO
;
Kyeung Min JOO
;
Yong JUNG
;
Juyoun JIN
;
Yonghyun KIM
;
Su Jin YOON
;
Seung Ho CHOI
;
Ho Jun SEOL
;
Woong Yang PARK
;
Do Hyun NAM
Author Information
1. Department of Neurosurgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea. nsnam@skku.edu
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
carcinoma, non-small-cell lung;
DNA damage;
gene expression profiling;
microarray analysis;
radiation;
tumor suppressor protein p53
- MeSH:
Adaptor Proteins, Signal Transducing/genetics;
Cell Cycle Proteins/genetics;
Cell Line, Tumor;
Cell Survival/radiation effects;
DNA Damage/*radiation effects;
DNA Repair Enzymes/genetics;
DNA-Binding Proteins/genetics;
Gene Expression Profiling;
Gene Expression Regulation, Neoplastic/*radiation effects;
Humans;
Lung Neoplasms;
Radiation Tolerance/genetics;
Signal Transduction
- From:Experimental & Molecular Medicine
2011;43(7):419-426
- CountryRepublic of Korea
- Language:English
-
Abstract:
Radiation is the most useful treatment modality for cancer patients. It initiates a series of signal cascades such as DNA damage response (DDR) signaling for repairing damaged DNA, arresting the cell cycle, and inducing cell death. Until now, few genes have been found to be regulated by radiation, which explains the molecular mechanisms of cellular responses to radiation. Although the transcriptional changes caused by radiation have been widely investigated, little is known about the direct evidence for the transcriptional control of DDR-related genes. Here, we examined the radiosensitivity of two non-small cell lung cancer cell lines (H460 and H1299), which have different p53 status. We monitored the time-dependent changes of 24 DDR-related gene expressions via microarray analysis. Based on the basal expression levels and temporal patterns, we further classified 24 DDR-related genes into four subgroups. Then, we also addressed the protein levels of several DDR-related genes such as TopBP1, Chk1 and Chk2, confirming the results of microarray analysis. Together, these results indicate that the expression patterns of DDR-related genes are associated with radiosensitivity and with the p53 statuses of H460 and H1299, which adds to the understanding of the complex biological responses to radiation.
