Discovery of the serum biomarker proteins in severe preeclampsia by proteomic analysis.
10.3858/emm.2011.43.7.047
- Author:
Jisook PARK
1
;
Dong Hyun CHA
;
Soo Jae LEE
;
Young Nam KIM
;
Young Hwan KIM
;
Kwang Pyo KIM
Author Information
1. Department of Molecular Biotechnology, Institute of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Korea. kpkim@konkuk.ac.kr
- Publication Type:Research Support, Non-U.S. Gov't ; Original Article
- Keywords:
alpha2HS-glycoprotein;
biomarkers;
pre-eclampsia;
spectrometry, mass, electrospray ionization;
proteomics;
serum
- MeSH:
Adult;
Alpha-Globulins/metabolism;
Amino Acid Sequence;
Biological Markers/blood;
Blood Proteins/*analysis;
Case-Control Studies;
Female;
Humans;
Insulin-Like Growth Factor Binding Proteins/blood;
Molecular Sequence Data;
Pre-Eclampsia/*blood/diagnosis;
Pregnancy;
Proteome/*analysis;
Retinol-Binding Proteins, Plasma/metabolism;
alpha-2-HS-Glycoprotein/metabolism
- From:Experimental & Molecular Medicine
2011;43(7):427-435
- CountryRepublic of Korea
- Language:English
-
Abstract:
Preeclapsia (PE) is a severe disorder that occurs during pregnancy, leading to maternal and fetal morbidity and mortality. PE affects about 3-8% of all pregnancies. In this study, we conducted liquid chromatographymass spectrometry/mass spectrometry (LC-MS/MS) to analyze serum samples depleted of the six most abundant proteins from normal and PE-affected pregnancies to profile serum proteins. A total of 237 proteins were confidently identified with < 1% false discovery rate from the two groups of duplicate analysis. The expression levels of those identified proteins were compared semiquantitatively by spectral counting. To further validate the candidate proteins with a quantitative mass spectrometric method, selective reaction monitoring (SRM) and enzyme linked immune assay (ELISA) of serum samples collected from pregnant women with severe PE (n = 8) or normal pregnant women (n = 5) was conducted. alpha2-HS-glycoprotein (AHSG), retinol binding protein 4 (RBP4) and alpha-1-microglobulin/bikunin (AMBP) and Insulin like growth factor binding protein, acid labile subunit (IGFBP-ALS) were confirmed to be differentially expressed in PE using SRM (P < 0.05). Among these proteins, AHSG was verified by ELISA and showed a statistically significant increase in PE samples when compared to controls.
