Two-sample bidirectional Mendelian randomization analysis of the causal relationship between gut microbiota and sepsis
10.3969/j.issn.1008-9691.2023.05.003
- VernacularTitle:两样本双向孟德尔随机化分析肠道微生物与脓毒症的因果关系
- Author:
Changhong MIAO
1
,
2
,
3
;
Xinyi XU
;
Lu XIAO
;
Jin WANG
;
Fangying WU
;
Kuang CHEN
Author Information
1. 天津中医药大学第一附属医院,天津 300381
2. 国家中医针灸临床医学研究中心,天津 300381
3. 天津中医药大学,天津 301617
- Keywords:
Mendelian randomization;
Gut microbiota;
Sepsis;
Causal relationship
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2023;30(5):523-528
- CountryChina
- Language:Chinese
-
Abstract:
Objective To delve into the causal relationship between 211 gut microbiota and sepsis employing bidirectional Mendelian randomization(MR).Methods The gut microbiota genome-wide association study(GWAS)data from the Microbiome Genetics Consortium(MiBioGen,n = 18 340)and sepsis GWAS data from the FinnGen(n = 286 146)were harnessed for this study.Initially,single nucleotide polymorphisms(SNP)significantly associated with the relative abundance of 211 gut microbiota taxa were identified as instrumental variables using predefined selection criteria.The primary analytical approach was characterized by the application of inverse variance weighting(IVW),with the effect measure represented by the odds ratio(OR)to assess the results of MR.To ensure precision and reliability,analyses were conducted,including leave-one-out analysis,heterogeneity testing,and tests for pleiotropy at both coherent and incoherent levels.Results The increased risk of sepsis was associated with the elevated abundance of Collinsella[OR = 1.28,95%confidence interval(95%CI)was 1.06-1.56,P = 0.01]and Ruminococcus(OR = 1.19,95%CI was 1.05-1.35,P = 0.005).Furthermore,a protective effect against the development of sepsis was observed in association with the increased abundance of Prevotella(OR = 0.88,95%CI was 0.79-0.97,P = 0.01)and Firmicutes(OR = 0.86,95%CI was 0.75-0.996,P = 0.04).No obvious heterogeneity and irrelevant level pleiotropy were detected.Conclusion Collinsella and Ruminococcus increase the risk of sepsis,while Prevotella and Firmicutes have protective effects against sepsis.
