Relationship between ITGA3 expression and immune cell infiltration in colorectal cancer
10.12354/j.issn.1000-8179.2023.20231043
- VernacularTitle:IGA3表达与结直肠癌免疫细胞浸润的关系
- Author:
Xiao LIU
1
;
Yanfeng XI
;
Peng BU
;
Guohai ZHAO
;
Peiyu JIN
;
Yuting FENG
;
Wei CUI
;
Jing XU
Author Information
1. 山西医科大学基础医学院病理教研室(太原市 030001)
- Keywords:
colorectal cancer(CRC);
immune infiltration;
CD8+T cell;
cell immune checkpoints
- From:
Chinese Journal of Clinical Oncology
2023;50(23):1196-1202
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the relationship between integrin ɑ3(ITGA3)expression and immune cell infiltration in colorectal cancer(CRC).Methods:Bioinformatic methods were used to analyze ITGA3 mRNA expression in pan-cancer and CRC tissues,as well as its associ-ation with CRC prognosis.The correlation between ITGA3 and tumor-infiltrating immune cells was also investigated.In total,233 cases of CRC diagnosed at Shanxi Provincial Cancer Hospital between January and December 2021 were included,and ITGA3,CD8,CD163,FOXP3,PD-L1,CTLA-4,and PD-1 expression in CRC tissues were determined by immunohistochemistry(IHC)to analyze the relationship between ITGA3 and infiltrating immune cells and immune checkpoints.Results:Bioinformatics analysis showed elevated ITGA3 mRNA levels in CRC.High ITGA3 expression was associated with PFS(P＜0.05).Univariate and multifactorial analyses showed that age and stage were significantly cor-related with prognosis(P＜0.05).In addition,ITGA3 upregulation was closely correlated with multiple immune cell infiltration levels in CRC.Furthermore,IHC results showed that ITGA3 expression in CRC tissues was significantly higher than that in adjacent normal tissues(P＜0.05).ITGA3 expression was associated with lymph node metastasis(P＜0.05)and correlated with the expression of immune markers,such as CD8+T-cells,PD-L1,and CTLA-4(P＜0.05).Conclusions:ITGA3 is highly expressed in CRC,which is closely related to immune cell infiltration and may regulate the tumor immune microenvironment,which provides a new idea for clinical treatment and a potential new independent predictive marker.
