Role of Ca2+in electromagnetic field regulation on osteoblast proliferation and differentiation
10.3969/j.issn.1005-202X.2024.01.014
- VernacularTitle:Ca2+在电磁场调控成骨细胞增殖与分化中的作用
- Author:
Guangwei ZHANG
1
;
Zhuowen LIANG
;
Zhi YANG
Author Information
1. 陕西中医药大学第二临床医学院,陕西咸阳 712046
- Keywords:
electromagnetic field;
osteoblast;
calcium ion;
cell proliferation;
cell differentiation
- From:
Chinese Journal of Medical Physics
2024;41(1):95-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of electromagnetic field(EMF)on thechange of Ca2+ in osteolbast from the qualitative and quantitative perspectives,and try to identify the role of Ca2+in EMF regulation on osteoblast proliferation and differentiation.Methods A platform was established for generating sine EMF with a frequency of 38.7 Hz and a strength of 1.5 mT.The MC3T3-E1 osteoblasts were randomly divided into control group and experimental group(EMF intervention for 8 h per day).CCK8 was used to detect osteoblast proliferation,ALP staining to detect osteoblast differentiation,and Ca2+fluorescence probes and flow cytometer to detect the Ca2+concentration in osteoblasts.Results CCK8 result showed that EMF intervention for 48,72,96 and 120 h could significantly promote osteoblast proliferation.After 14 days of EMF intervention,the positive expression of ALP was significantly higher in EMF group than in control group.Ca2+fluorescent staining and flow cytometry results revealed that EMF intervention could increase the Ca2+in osteoblasts.Conclusion The EMF-induced upregulation of Ca2+ signal in osteoblasts may be closely related to the promotions of osteoblast proliferation and differentiation by EMF,but which Ca2+-related biosignaling pathways are involved in the EMF promoting osteoblast proliferation and differentiation remains to be further investigated.
