Differential expression of inflammatory proteins in diabetic skin ulcers and ordinary skin ulcers
10.3760/cma.j.cn431274-20240304-00363
- VernacularTitle:糖尿病皮肤溃疡与普通皮肤溃疡中炎症蛋白差异性表达的研究
- Author:
Wu XIONG
1
;
Youyuan HE
;
Xi ZHANG
;
Jianda ZHOU
;
Jia CHEN
;
Xiaoling ZOU
;
Sijia ZHAO
;
Xingxing ZHONG
;
Yutan CAO
;
Wenjing QU
Author Information
1. 湖南中医药大学第一附属医院烧伤整形外科，长沙 410007
- Keywords:
Diabetes mellitus;
Skin ulcer;
Inflammatory proteins;
Pro-inflammatory factor;
Anti-inflammatory factor
- From:
Journal of Chinese Physician
2024;26(3):331-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study and screen the differential expression of inflammatory proteins in diabetes skin ulcers and common skin ulcers, so as to provide experimental basis for further research on anti-inflammatory and healing drug targets of diabetes skin ulcers.Methods:The tissues of 11 patients with diabetes skin ulcer, 12 patients with common skin ulcer and 11 patients with normal skin were collected from the First Hospital of Hunan University of Chinese Medicine. The levels of inflammatory protein Toll like receptor 4 (TLR4), nuclear factor κB (NF-κB), pro-inflammatory factor interferon -γ (IFN -γ), tumor necrosis factor - α (TNF -α), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-1β (IL-1β), macrophage chemotactic protein-1 (MCP-1), anti-inflammatory factors epidermal growth factor (EGF), interleukin-4 (IL-4), and interleukin-10 (IL-10) were detected in three groups of tissues using enzyme-linked immunosorbent assay (ELISA).Results:Compared with normal tissues, the concentrations of TLR4, NF-κB, IFN -γ, TNF -α, IL-1β, IL-6, IL-8, MCP-1 and EGF in common ulcer skin tissues and diabetes ulcer tissues were higher, and the concentrations of IL-10 were lower, with statistically significant differences (all P<0.05); Compared with the normal tissue, the concentration of IL-4 in diabetes ulcer tissue was lower, the difference was statistically significant ( P<0.05); Compared with ordinary ulcer skin tissue, the concentrations of TLR4, NF-κB and MCP-1 in diabetes ulcer tissue were higher, and the concentrations of IL-4 were lower, with statistically significant differences (all P<0.05). Conclusions:The skin ulcer in diabetes patients will have inflammatory reaction, and high glucose promotes the inflammatory reaction of skin ulcer, which may be related to the abnormal expression of TLR4, NF-κB, MCP-1 and IL-4. TLR4/NF-κB signal pathway and inflammatory factors MCP-1 and IL-4 may be the target of the inflammation regulation of diabetes skin ulcer.
