Analysis of volatile constituents in different parts of Huai chrysanthemum by GC-MS combined with stoichiometry
10.12173/j.issn.1008-049X.202401060
- VernacularTitle:基于GC-MS和化学计量学的怀菊不同部位挥发性成分差异分析
- Author:
Mengzhen GUO
1
,
2
;
Meng LI
;
Xiaoyan DENG
;
Shuyan LIU
;
Xiaolan WANG
;
Xiaoya SUN
;
Jingke ZHANG
;
Xiaoke ZHENG
;
Weisheng FENG
Author Information
1. 河南中医药大学药学院(郑州 450046)
2. 河南省中药开发工程技术研究中心(郑州 450046)
- Keywords:
Huai chrysanthemumin;
Different parts;
Volatile oil;
Gas chromatography-mass spectrometry;
Chemometrics
- From:
China Pharmacist
2024;27(2):209-219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and identify the volatile constituents in different parts(flowers,stems and leaves)of Huai chrysanthemumin,and to lay a theoretical foundation for the comprehensive utilization for it.Methods The volatile oil in different parts of Huai chrysanthemumin were extracted by hydrodistillation,respectively.Their constituents were analyzed by gas chromatography-mass spectrometry(GC-MS).The compounds were identified by library search and literature screening.The relative percentage of each compound was obtained by the area normalization method.The differences in their chemical compositions were analyzed by Venn diagram,principal component analysis(PCA)and cluster heat map analysis.Results A total of 62 volatile chemical components were identified from different parts of Huai chrysanthemumin,including monoterpenes,sesquiterpenes,and their derivatives,as well as a small amount of aliphatic compounds.32,42 and 40 volatile components were detected from the flowers,stems and flowers,respectively.Furthermore 17 volatile components were shared by three parts,whereas 5,6 and 16 volatile components were unique to the flowers,stems and leaves,respectively.The results of stoichiometric analysis showed that both PCA and cluster heat map analysis could separate the flowers,stems and leaves,and their volatile components were different.Conclusion The types and contents of the volatile oil in the stems,leaves and flowers of Huai chrysanthemumin have certain variability,which provide a scientific basis for the further medicinal or industrial exploitation of different parts of Huai chrysanthemumin.