Study on the correlation between Xiaochengqitang pieces(decoction and granules)based on ultra high performance liquid chromatography fingerprint
10.12173/j.issn.1008-049X.202209317
- VernacularTitle:小承气汤饮片(水煎液、颗粒)的UPLC指纹图谱相关性研究
- Author:
Jiangping CHEN
1
;
Shan WEN
;
Guihai DENG
;
Qiuyi MO
;
Wenting SHI
;
Caiyue QIU
;
Yun LU
Author Information
1. 广东一方制药有限公司/广东省中药配方颗粒企业重点实验室(广东佛山 528244)
- Keywords:
Xiaochengqitang pieces(decoction and granules);
Ultra high performance liquid chromatography fingerprint;
Correlation
- From:
China Pharmacist
2024;27(1):46-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the correlation of an ultra high performance liquid chromatography(UPLC)fingerprint of Xiaochengqitang pieces(decoction and granules).Methods The UPLC method was used to establish the fingerprint of 15 batches of Xiaochengqitang pieces(decoction and granules).The correlation of the three UPLC fingerprints was evaluated by similarity analysis,pearson correlation analysis,cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA).Results UPLC fingerprints of 15 batches of Xiaochengqitang pieces(decoction and granules)determined 16 common peaks,and 14 peaks were identified.The similarity of the fingerprints of the 15 batches of Xiaochengqitang pieces(decoction and granules)with the corresponding control fingerprints was greater than 0.90,and the similarity of the three control fingerprints was greater than 0.88.The results of pearson correlation analysis showed that 8 common peaks in Xiaochengqitang pieces(decoction and granules)had a very significant positive correlation.The results of CA showed that the properties of Xiaochengqitang decoction and granules were more similar.The results of PCA showed that the principal components with 4 eigenvalues greater than 1 contained 88％of the information of the original data.OPLS-DA screened 7 differential markers with variable importance projection value greater than 1.Conclusion The main chemical compositions of Xiaochengqitang pieces(decoction and granules)are consistent,which can provide data support for the quality control and clinical use of Xiaochengqitang compound preparation.
