Quality evaluation of Tongshu gel paste based on multi-index component quantification
10.12173/j.issn.1008-049X.202310044
- VernacularTitle:基于多指标成分定量的痛舒凝胶贴膏质量评价研究
- Author:
Tong GUAN
1
;
Xue YANG
;
Jing LIU
;
Xiufeng YU
;
Yanhong WANG
Author Information
1. 黑龙江中医药大学药学院(哈尔滨 150040)
- Keywords:
Tongshu gel paste;
Thin layer chromatography;
High performance liquid chromatography;
Quality evaluation
- From:
China Pharmacist
2024;27(1):27-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the quality of Tongshu gel paste based on thin layer chromatography(TLC)identification and high performance liquid chromatography(HPLC)quantitative analysis of multi-index components.Methods Qualitative identification of tetrahydropalmatine,paeoniflorin,tetrandrine,linderane,Rhizoma atractylodis and Fructus aurantii in the modified Tongshu gel paste was carried out by TLC.The main active contents of tetrahydropalmatine,paeoniflorin,tetrandrine and linderane in the preparation were determined by HPLC.Results The TLC spots of the six herbs in the prescription were clear and negative without interference.The linear ranges of tetrahydropalmatine,paeoniflorin,tetrandrine and linderane were 4.30-272.50 μg/mL(r=0.999 9),21.10-1 350.00 μg/mL(r=0.999 8),4.10-262.40 μg/mL(r=0.999 8)and 3.60-232.50 μg/mL(r=0.998 6),respectively.The range of average recovery for each component was 98.96％-99.52％,and all RSDs were less than or equal to 1.54％(n=9).Conclusion The multi-index TLC qualitative identification and HPLC quantitative method established by research have high specificity,simple operation and can be used for quality evaluation of the improved formulation of this prescription.
