Comprehensive quality evaluation of Patrina scabiosaefolia Fisch based on HPLC multi-component quantitative control and PCA,OPLS-DA combined with gray correlation analysis
10.12173/j.issn.1008-049X.202309064
- VernacularTitle:黄花败酱HPLC多组分定量控制及PCA、OPLS-DA联合GRA法综合质量评价
- Author:
Li-Xin LU
1
;
Lei ZHENG
;
Zhi-Ming LI
;
Chao SONG
Author Information
1. 山东省立第三医院药学部(济南 250000)
- Keywords:
Patrina scabiosaefolia Fisch;
High performance liquid chromatography;
Grey relational analysis;
Principal component analysis;
Orthogonal partial least squares-discriminant analysis;
Comprehensive evaluation
- From:
China Pharmacist
2023;26(12):510-518
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the simultaneous determination of hederagenin,oleanolic acid,ursolic acid,scabioside C,protocatechuic acid,chlorogenic acid,caffeic acid,aesculetin,scopoletin,rutoside,quercetin,kaempferol in Patrina scabiosaefolia Fisch,and to explore the application of principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA)and grey relational analysis(GRA)in the quality evaluation of Patrina scabiosaefolia Fisch.Methods The HPLC method was used with the Waters Atlantis T3 C18 column(250 mm×4.6 mm,5 μm).The mobile phase was acetonitrile-0.1% phosphate acid solution with gradient elution.The detection wavelengths were 210,260 and 360 nm.The overall quality of Patrina scabiosaefolia Fisch was comprehensively evaluated by PCA,OPLS-DA and GRA analysis of the results of multi-component content.Results The results of methodological verification of external standard method met the requirements.The 12 components had good linear relationships within their respective ranges(r>0.999 0),and the average recovery rate were 96.82% -100.16% (RSD<2.0% ,n=9).PCA and OPLS-DA results showed that oleanolic acid,ursolic acid,chlorogenic acid and quercetin were the main potential markers affecting the quality of Patrina scabiosaefolia Fisch.The relative correlation degree of GRA were 0.380 6-0.571 4,and there was a certain difference between batches of Patrina scabiosaefolia Fisch.Conclusion The HPLC method can simultaneously determine the 12 components in Patrina scabiosaefolia Fisch,which is simple and practical,and PCA,OPLS-DA and GRA can be used to evaluate the quality of Patrina scabiosaefolia Fisch.
