Study on extraction technology for Sihuang Shaoshang ointment based on HPLC fingerprint and principal component analysis
10.12173/j.issn.1008-049X.202208130
- VernacularTitle:基于HPLC指纹图谱与主成分分析相结合的四黄烧伤膏提取工艺研究
- Author:
Chang-He LIU
1
,
2
;
Dan-Dan JIAN
;
Sheng-Hu CHEN
;
Hua-Ni LI
;
Yan-Yan WANG
;
Xue-Xia ZHANG
Author Information
1. 河南省中医药研究院(郑州 450004)
2. 河南省中药制剂工程技术研究中心(郑州 450004)
- Keywords:
Sihuang Shaoshang ointment;
Orthogonal test;
Principal component analysis;
Fingerprint;
Extraction technology
- From:
China Pharmacist
2023;26(12):374-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize extraction technology for Sihuang Shaoshang ointment by orthogonal test,and establish the HPLC fingerprint of Sihuang Shaoshang ointment to provide references for the quality evaluation of the preparation.Methods Taking Sihuang Shaoshang ointment as the model drug,the fingerprints were established by HPLC method,the common peak areas were analyzed by principal component analysis,and the total factor scores were used as the evaluation index.The technological parameters such as extraction times,extraction time and liquid-solid ratio were optimized by orthogonal test,and the optimal extraction process parameters were screened out.Results The common mode of fingerprint was set up with 12 common peaks and the two principle components with the accumulative contribution rate of 84.751% extracted by principal component analysis were screened out to calculate the comprehensive scores of 9 samples.The optimal extraction process was as follows:10-fold amount of water,extracting 3 times,and extracting 2 h for each time.Conclusion The established method is simple and effective,which can provide a reference for the extraction process optimization of Sihuang Shaoshang ointment.
