Determination of illegally added metolazone in tablet candy by Captiva EMR-Lipid technology combined with UPLC-Q-TOF/MS
10.12173/j.issn.1008-049X.202208140
- VernacularTitle:Captiva EMR-Lipid技术结合UPLC-Q-TOF/MS测定压片糖果中非法添加的美托拉宗
- Author:
Hua WANG
1
;
Chan CHEN
;
Hong-Hong LIU
;
Shan-Shan HE
;
Da-Long LUO
Author Information
1. 梧州市食品药品检验所(广西梧州 543000)
- Keywords:
Metolazone;
Captiva EMR-Lipid solid phase extraction;
Quadrupole time of flight mass spectrometry;
Tablet candy;
Illegally added drugs
- From:
China Pharmacist
2023;26(11):355-360
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for rapid screening and quantifying the illegal addition of metolazone in tablet candy by ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF/MS).Methods The component in samples was extracted with acetonitrile,then purified by Captiva EMR-Lipid purification column,detected by UPLC-Q-TOF/MS with Agilent RRHD Eclipse Plus C18 chromatographic column(100 mm×2.1 mm,1.8μm)under Targeted MS/MS mode.Results The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 50-1 000 ng·mL-1 for metolazone(r=0.999 0);the value of detection limit and quantification limit was found to be 1.0 μg.g1 and 2.5 μg·g-1.The average recovery rate was 98.15％(RSD=2.2％,n=18).Conclusion The method had the advantages of simple operation,qualitative and quantitative accuracy.It could be applied to daily inspection.
