Multi-component comprehensive quality evaluation of Tongjing ointment
10.12173/j.issn.1008-049X.202305012
- VernacularTitle:痛经软膏的多成分综合质量评价
- Author:
Ling-Chao RAN
1
;
Jin-Ying FENG
;
Zhi-Ming LI
;
Kang-Kang JI
Author Information
1. 山东省立第三医院药学部(济南 250000)
- Keywords:
Tongjing ointment;
High performance liquid chromatography-quantitative analysis of multi-components by a single marker;
Chemometrics;
Grey relational analysis;
Comprehensive evaluation
- From:
China Pharmacist
2023;26(11):319-331
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for comprehensive quality evaluation of Tongjing ointment by multi-component quantification combined with chemometrics and grey correlation analysis(GRA).Methods With curcumin as the internal reference substance,HPLC-QAMS method was used to simultaneously determine the contents of limonin,evodiamine,rutaecarpine,bisdemethoxycurcumin,demethoxycurcumin,curcumin,6-gingerol,8-gingerol,10-gingerol,tetrahydropalmatine,dehydrocorydalin,dorydaline in Tongjing ointment.The quality of Tongjing ointment was evaluated by cluster analysis,principal component analysis,orthogonal partial least square discriminant analysis and GRA.Results The determination of 12 components manifested a good linear relationship in the range of mass concentration(r≥0.999 1).The average recovery was between 96.58％and 100.19％(RSD＜2.0％,n=9).There were no significant difference between the measured value of external standard method and the calculated value of HPLC-QAMS(P＞0.05).Tongjing ointment samples were classified into three eategories by chemometrics and it showed that curcumin,6-gingerol,bisdemethoxycurcumin,demethoxycurcumin,limonin and tetrahydropalmatine were the main potential markers affecting the quality of Tongjing ointment.GRA showed that the relative correlation degree was in the range of 0.317 3-0.624 8,and there were some differences in the quality of Tongjing ointment.Conclusion The established method can comprehensively evaluate the quality of Tongjing ointment.
