The clinical value of UPLC-MS/MS method for the determination of plasma and tissue isoniazid concentration in lymph node tuberculosis patients
10.12173/j.issn.1008-049X.202303215
- VernacularTitle:UPLC-MS/MS法测定淋巴结结核患者血浆和组织异烟肼浓度的临床价值研究
- Author:
Yu ZHANG
1
;
Xiao-Yu LIU
;
Feng XU
;
Huan ZHANG
;
Qiao-Yan DING
;
Li-Hua MA
;
Si-Si LI
;
Ming ZHOU
Author Information
1. 武汉市肺科医院药学部(武汉 430030)
- Keywords:
Lymph node tuberculosis;
Isoniazid;
Ultra performance liquid chromatography-mass spectrum/mass spectrum
- From:
China Pharmacist
2023;26(11):311-318
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between isoniazid concentration in plasma and lymph node tissue of patients with lymph node tuberculosis,and to explore its clinical value.Methods The basic information of patients with lymph node tuberculosis in our hospital and venous blood samples and neck lymph node tissue samples at different time points were collected.UPLC-MS/MS method was established and isoniazid concentration in plasma and neck lymph node tissue samples was quantitatively detected,and the correlation between isoniazid concentration in plasma and lymph node tissue was analyzed.Results The linear range of isoniazid blood concentration and lymph node tissue concentration were 0.25-16 μg·mL-1(r=0.999 8)and 2-128 μg·g-1(r=0.998 8),respectively.The precision,accuracy,and matrix effect of each quality control sample met the requirements.Plasma isoniazid concentration and lymph node tissue isoniazid concentration were significantly correlated at 10 min(rs=0.501 1,P=0.001),30 min(rs=0.402 8,P=0.005)and 60 min(rs=0.614 6,P=0.001)after intravenous infusion of isoniazid.The ratio of lymph node tissue isoniazid concentration to plasma isoniazid concentration was 1.46(0.62,3.55)mL·g-1 at 10 min.At 30 min,the ratio was 5.25(4.61,11.61)mL·g-1.At 60 min,the ratio was 6.62(4.42,10.78)mL·g-1.Conclusion The established UPLC-MS/MS method has good specificity,high sensitivity,accurac and precision.Monitoring plasma isoniazid concentration provides a reference for the rational use of isoniazid in patients with lymphatic tuberculosis.
