Effect of erianin on angiogenesis of glomerular endothelial cells in diabetic nephropathy model rats by regulating Slit2/Robo1 signaling pathway
10.3867/j.issn.1000-3002.2024.03.003
- VernacularTitle:毛兰素调节Slit2/Robo1信号通路对糖尿病肾病模型大鼠肾小球内皮细胞血管生成的影响
- Author:
Juyun ZHANG
1
;
Mianxiong CHEN
;
Binghong HUA
;
Xubiao MENG
Author Information
1. 中南大学湘雅医学院附属海口医院,海南 海口 570208
- Keywords:
erianin;
slit homolog 2 protein;
roundabout homolog 1;
diabetic nephropathy;
glomerular endothelial cells;
angiogenesis
- From:
Chinese Journal of Pharmacology and Toxicology
2024;38(3):177-182
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effect of erianin on the angiogenesis of glomerular endothelial cells in diabetic nephropathy(DN)rats and the role of slit homolog 2 protein(Slit2)/roundabout homolog 1(Robo1)consecutive signaling pathway.METHODS Rats were fed with high sugar and high fat feed for 8 weeks,before being intraperitoneally injected with streptozotocin solution(35 mg·kg-1)to prepare a DN rat model.DN rats were divided into the model group and model+erianin 10,20 and 40 mg·kg-1 groups,with 10 rats in each,while another 10 rats served as normal control group.The urine protein quantification kit was used to measure the 24 h urine protein level of rats in each group while the automatic biochemical analyzer was used to detect the fasting plasma glucose(FPG)and serum creatinine(Scr)levels of rats in each group.PAS staining was applied to observe the pathological changes in the renal tissue of rats in each group.Immunofluorescence was used to detect the expressions of platelet endo-thelial cell adhesion molecule-31(CD31)and podocalyxin(PCX)in kidney tissue of rats in each group.Western blot was adopted to detect the expressions of Slit2 and Robo1 proteins in the renal tissues of rats in each group.RESULTS Compared with normal control group,the CD31 protein expressions,FPG,Scr,24 h urine protein levels,and renal tissue Slit2 and Robo1 protein expressions were significantly increased in the model group(P<0.05).Pathological and immunofluorescence results suggested that rats in the model group developed many neoplastic glomerular capillaries,glomerular hypertrophy,and dilated mesangial areas,with non-tubular CD31 staining lacking adjacent PCX staining,and partial staining of tubular areas of CD31 lacking adjacent PCX staining.Compared with the model group,the CD31 glomerular endothelial area,FPG,Scr,24 h urine protein levels,and protein expressions of Slit2 and Robo1 in renal tissues were significantly reduced in the model+erianin 10,20 and 40 mg·kg-1 groups(P<0.05).Pathological and immunofluorescence results showed new glomerular capillaries,glomerular hypertrophy and dilatation of the thylakoid area were attenuated in rats,and CD31 tubular region staining was essentially adjacent to the PCX foot cell region staining in the model+erianin 10,20 and 40 mg·kg-1 groups.CONCLUSION Erianin may inhibit angiogenesis in glomerular endothelial cells of DN model rats by inhibiting the Slit2/Robo1 signaling pathway.
