Electroacupuncture alleviates orofacial allo-dynia and anxiety-like behaviors by regulating synaptic plasticity of the CA1 hippocampal region in a mouse model of trigeminal neuralgia
10.3867/j.issn.1000-3002.2023.z1.095
- Author:
Yuzhi JIA
1
;
Haitao LI
;
Guangming ZHANG
;
Hongyun WU
;
Sishuo ZHANG
;
Hongwei ZHI
;
Yahan WANG
;
Jingwen ZHU
;
Yifan WANG
;
Xiangqing XU
;
Caijun TIAN
;
Wenqiang CUI
Author Information
1. First College of Clinical Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250000,Shandong,China
- Keywords:
trigeminaing neuralgia;
anxiety;
elec-troacupuncture;
synaptic plasticity;
hippocampal CA1 region
- From:
Chinese Journal of Pharmacology and Toxicology
2023;37(z1):69-70
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate whether electroacupuncture(EA)ameliorates abnormal trigeminal neuralgia(TN)orofacial pain and anxiety-like behavior by altering synaptic plasticity in the hippocampus CA1.METHODS A mouse infraorbital nerve transection model(pT-ION)of neuropathic pain was established,and EA or sham EA was used to treat ipsilateral acu-puncture points(GV20-Baihui and ST7-Xia-guan).Golgi-Cox staining and transmission elec-tron microscopy(TEM)were administrated to observe the changes of synaptic plasticity in the hippocampus CA1.RESULTS Stable and per-sistent orofacial allodynia and anxiety-like behav-iors induced by pT-ION were related to changes in hippocampal synaptic plasticity.Golgi stain-ings showed a decrease in the density of dendritic spines,especially mushroom-type dendritic spines,in hippocampal CA1 neurons of pT-ION mice.TEM results showed that the density of synapses,membrane thickness of the postsynap-tic density,and length of the synaptic active zone were decreased,whereas the width of the synap-tic cleft was increased in pTION mice.EA attenu-ated pT-ION-induced orofacial allodynia and anx-iety-like behaviors and effectively reversed the abnormal changes in dendritic spines and syn-apse of the hippocampal CA1 region.CONCLU-SION EA modulates synaptic plasticity of hippo-campal CA1 neurons,and reduces abnormal oro-facial pain and anxiety-like behavior,providing evidence for a TN treatment strategy.
