Exploring the mechanism of Bazi Bushen capsule in delaying the senescence of mesen-chymal stem cells by network pharmacology method and verifying it in vitro
10.3867/j.issn.1000-3002.2023.z1.062
- Author:
Yaping ZHANG
1
,
2
;
Tongxing WANG
;
Junqing LIANG
;
Cong WEI
Author Information
1. Hebei University of Chinese Medicine,Shijia-zhuang 050000,China
2. National Key Laboratory for Innovation and Transformation of Luobing Theory,Shijiazhuang 050000,China
- Keywords:
Bazi Bushen capsule;
aging;
mesen-chymal stem cells;
cell cycle;
network pharmacol-ogy
- From:
Chinese Journal of Pharmacology and Toxicology
2023;37(z1):39-39
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the key mechanism of Bazi Bushen capsule(BZBS)in delaying the senescence of mesenchymal stem cells(MSCs)through network pharmacology and in vitro experiments.METHODS Network phar-macology was used to predict the mechanism targets of BZBS in delaying MSCs senescence.A MSCs senescence model induced by D-galac-tose(D-gal)was used to investigate the effect and mechanism of BZBS on MSCs senescence in vitro.RESULTS Network pharmacology analy-sis showed that BZSB could delay MSCs senes-cence.The experiment showed that BZBS could significantly improve the survival activity of the aged MSCs.It significantly reduced the positive rate of β-galactosidase staining and p16,p21 expression in aged MSCs,enhanced the ability of adipogenic differentiation and osteogenic differ-entiation,and increased expression of Nanog,OCT4 and SOX2 in senescent MSCs.CONCLU-SIONS Network pharmacology and in vitro cell experiments verified that BZBS could delay MSCs senescence.
