Applications of activity-based protein profiling and affinity-based protein profiling in pharmacology and toxicology research
10.3867/j.issn.1000-3002.2023.12.008
- VernacularTitle:基于活性和基于亲和性的蛋白质表达谱在药理毒理学研究中的应用进展
- Author:
Jiasheng CHEN
1
,
2
;
Xinyue ZHANG
;
Junhong LIU
;
Wenqi MENG
;
Ling ZHANG
Author Information
1. 徐州医科大学药学院,江苏徐州 221004
2. 海军军医大学海军医学系,上海 200433
- Keywords:
activity-based protein profiling;
affinity-based protein profiling;
target identification;
drug off target;
toxic compound
- From:
Chinese Journal of Pharmacology and Toxicology
2023;37(12):951-958
- CountryChina
- Language:Chinese
-
Abstract:
Activity-based protein profiling(ABPP)and affinity-based protein profiling(AfBPP)are reliable chemical proteomics techniques that exhibit significant advantages in identifying the direct acting targets of small molecule drugs and toxicants,which can help researchers understand the pharmaco-logical and toxicological mechanisms of active small molecules.This paper introduces ABPP and AfBPP technologies and summarizes the applications of this technique in drug off-target effect and target iden-tification of toxicants in recent years,such as drug off-target effect of crenolanib,BIA 10-2474,orlistat and target identification of VX,fenitrothion,acrolein.It is hoped that this review will give readers a better idea of ABPP/AfBPP and offer a line of thinking for researchers in the fields of pharmacology,toxicology and chemical biology.
