Sea-buckthorn slows down-regulation of CYP 2C in mice with liver injury by BCG-induced via PXR/NF-κB pathway
10.12360/CPB202306073
- VernacularTitle:沙棘经PXR/NF-κB通路减缓免疫性肝损伤小鼠肝脏CYP2C下调
- Author:
Pei-Pei HAO
1
;
Ying-Ying CAO
;
Hui-Qiong ZOU
;
Rui-Feng DING
;
Xue-Feng BAI
;
Yong-Zhi XUE
Author Information
1. 内蒙古科技大学包头医学院
- Keywords:
BCG vaccine;
sea-buckthorn;
NF-κB;
PXR;
CYP2C;
hepatitis B
- From:
Chinese Pharmacological Bulletin
2023;39(12):2320-2324
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore whether sea-buckthorn slows down-regulation of hepatic cytochrome P450 2C(CYP 2C)in mice with liver injury by BCG vaccine(BCG)-induced via PXR/NF-κB pathway.Methods The mouse model of liver injury was induced by a single tail vein injection of BCG 125 mg·kg-1 for hepatitis B research,and the mice were randomly di-vided into control group,BCG group,BCG+sea-buckthorn granules(SG)group(gavage 50,100,200 mg·kg-1,twice a day),BCG+PCN group(intraper-itoneal injection 100 mg·kg-1,once a day).The levels of serum transaminase and TNF-α and IL-1β in liver tissue were detected by ELISA.The nuclear pro-tein expression NF-κB p65 and the total protein expres-sion of pregnane X receptor(PXR),CYP2C in liver were detected by Western blot.Liver pathological changes were observed by HE staining.Results Sea-buckthorn inhibited overexpression of TNF-α,IL-1βand NF-κB p65,alleviated the down-regulation of CYP2C and PXR protein expression,and improved liv-er pathology and serum transaminase in a dose-depend-ent manner.After intervention with PCN,a mouse specific agonist of PXR,it was similar to the high dose group of SG.Conclusion Sea-buckthorn slows down-regulation of CYP2C in mice with liver injury by BCG-induced via PXR/NF-κB pathway.
