Effects of long noncoding RNA-NRON on apoptosis following myocardial infarction in mice
10.12007/j.issn.0258-4646.2024.03.005
- VernacularTitle:长链非编码RNA-NRON对小鼠心肌梗死后细胞凋亡的作用机制研究
- Author:
Han GAO
1
;
Chunjing ZHANG
;
Shuyan LI
;
Yan SHI
;
Hongyan GUO
;
Chao YANG
Author Information
1. 齐齐哈尔医学院医学技术学院生物化学教研室,黑龙江 齐齐哈尔 161006
- Keywords:
long noncoding RNA-NRON;
myocardial infarction;
voltage dependent anion channel;
apoptosis
- From:
Journal of China Medical University
2024;53(3):218-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of long noncoding RNA(lncRNA)-NRON on apoptosis following myocardial infarc-tion(MI)in mice.Methods The C57BL/6 mice were randomly divided into four groups:sham operation(Sham)group,MI group,MI combined with lncRNA-NRON interference lentivirus(MI+shNRON)group,and MI combined with the negative control(NC)lentivirus(MI+NC)group.The expression of lncRNA-NRON was detected using real-time PCR.In addition,the pathology of the myocardial tissue injury was analyzed using HE staining,the myocardial infarction size was examined using TTC staining,and the extent of apoptosis was assessed using the TUNEL assay,respectively.The RPISeq database was used to predict the probability of interaction between lncR-NA-NRON and the voltage-dependent anionic channel protein(VDAC).The effect of lncRNA-NRON on the expression of VDAC protein was detected using Western blotting.Results The lncRNA-NRON expression was significantly increased in the MI group,and the tar-geted knockdown of lncRNA-NRON resulted in alleviation of the pathological myocardial tissue injury,reduction in the myocardial infarc-tion area,and inhibition of apoptosis.The probability of interaction between lncRNA-NRON and VDAC reached 0.9,indicating a high probability of their association.Additionally,lncRNA-NRON could regulate the protein expression of VDAC.Conclusion Knockdown of lncRNA-NRON could reduce the occurrence of myocardial injury following myocardial infarction.This effect may be attributable to a spe-cific mechanism wherein lncRNA-NRON affects the process of apoptosis by binding to VDAC,consequently suppressing its expression.
